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The following table shows the results of the chromatographic analysis of the 4 components separated isocratically using an inverted phase column with a length of 10 cm, a mobile phase flow rate of 0.2 cm/s, and a UV-Vis detector. Non retained Analite A Analite B species 0,1 PARAMETER Analite C Analite D www PEAK HEIGHT (au) 2,3 0,22 0,52 1,16 ELUSION TIME (s) 53 67 102 147 217 WIDE PEAK BASE 7 10 15 22 33 (s) a. Assuming the same fourth molar absorptivity of analytes, which analyte has the highest concentration? b. What is the selectivity factor between analytes B and C? c. Which component has the best efficiency? d. What is the theoretical plate height on the column used? e. What is the resolution between analytes A and B? does it qualify for quantitative analysis?

The following table shows the results of the chromatographic analysis of the 4 components separated isocratically using an inverted phase column with a length of 10 cm, a mobile phase flow rate of 0.2 cm/s, and a UV-Vis detector. Non retained Analite A Analite B species 0,1 PARAMETER Analite C Analite D www PEAK HEIGHT (au) 2,3 0,22 0,52 1,16 ELUSION TIME (s) 53 67 102 147 217 WIDE PEAK BASE 7 10 15 22 33 (s) a. Assuming the same fourth molar absorptivity of analytes, which analyte has the highest concentration? b. What is the selectivity factor between analytes B and C? c. Which component has the best efficiency? d. What is the theoretical plate height on the column used? e. What is the resolution between analytes A and B? does it qualify for quantitative analysis?

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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The following table shows the results of the chromatographic analysis of the 4 components separated isocratically using an inverted phase column with a length of 10 cm, a mobile phase flow rate of 0.2 cm/s, and a UV-Vis detector. PARAMETER Non retained Analite A Analite B Analite C Analite D species 0,1 PEAK HEIGHT (au) 2,3 0,22 0,52 1,16 ELUSION TIME (s) 53 67 102 147 217 WIDE PEAK BASE 7 10 15 22 33 (s) a. Assuming the same fourth molar absorptivity of analytes, which analyte has the highest concentration? b. What is the selectivity factor between analytes B and C? c. Which component has the best efficiency? d. What is the theoretical plate height on the column used? e. What is the resolution between analytes A and B? does it qualify for quantitative analysis?
Transcribed Image Text:The following table shows the results of the chromatographic analysis of the 4 components separated isocratically using an inverted phase column with a length of 10 cm, a mobile phase flow rate of 0.2 cm/s, and a UV-Vis detector. PARAMETER Non retained Analite A Analite B Analite C Analite D species 0,1 PEAK HEIGHT (au) 2,3 0,22 0,52 1,16 ELUSION TIME (s) 53 67 102 147 217 WIDE PEAK BASE 7 10 15 22 33 (s) a. Assuming the same fourth molar absorptivity of analytes, which analyte has the highest concentration? b. What is the selectivity factor between analytes B and C? c. Which component has the best efficiency? d. What is the theoretical plate height on the column used? e. What is the resolution between analytes A and B? does it qualify for quantitative analysis?
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