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The figure shows an example of a thermal denaturation plot. The melting temperature (Tm) is the midpoint of the temperature range over which denaturation occurs. Percent unfolded 100 80 60 40 20 0 20 Tm 40 60 Temperature (°C) 80 100

The figure shows an example of a thermal denaturation plot. The melting temperature (Tm) is the midpoint of the temperature range over which denaturation occurs. Percent unfolded 100 80 60 40 20 0 20 Tm 40 60 Temperature (°C) 80 100

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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The figure shows an example of a thermal denaturation plot. The melting temperature (T) is the midpoint of the temperature range over which denaturation occurs. Percent unfolded 100 80 60 40 20 0 20 Tm E 40 60 Temperature (°C) 80 100 Suppose that a Met residue buried in the folded, hydrophobic core of T4 lysozyme is replaced by mutation with a Lys residue.
Transcribed Image Text:The figure shows an example of a thermal denaturation plot. The melting temperature (T) is the midpoint of the temperature range over which denaturation occurs. Percent unfolded 100 80 60 40 20 0 20 Tm E 40 60 Temperature (°C) 80 100 Suppose that a Met residue buried in the folded, hydrophobic core of T4 lysozyme is replaced by mutation with a Lys residue.
How would the Met to Lys mutation affect the thermal denaturation of lysozyme at pH 3.0? It will destabilize the protein and lower the Tm, shifting the curve to the left. It will stabilize the protein and raise the Tm, shifting the curve to higher temperatures. It will not change the T at pH 3.0 because the Lys side chain pK, is 10.53. It will not affect stability, but it will decrease the Tm and shift the curve to lower temperatures.
Transcribed Image Text:How would the Met to Lys mutation affect the thermal denaturation of lysozyme at pH 3.0? It will destabilize the protein and lower the Tm, shifting the curve to the left. It will stabilize the protein and raise the Tm, shifting the curve to higher temperatures. It will not change the T at pH 3.0 because the Lys side chain pK, is 10.53. It will not affect stability, but it will decrease the Tm and shift the curve to lower temperatures.
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