The figure below is an absorption spectrum for a food dye molecule. What wavelength would be best used to measure absorbance in a spectrophotometer? And what color does the solution appear to be? 0.8 FD&C Red No. 3 0.7- "Erythrosine" CHJ,O, •2 Na 0.6- MW = 897.92 g/mole 0.5- но 0.4- 0.3- ENO 0.2 0.1 0. 400 450 500 550 600 650 700 Absorbance

The figure below is an absorption spectrum for a food dye molecule. What wavelength would be best used to measure absorbance in a spectrophotometer? And what color does the solution appear to be? 0.8 FD&C Red No. 3 0.7- "Erythrosine" CHJ,O, •2 Na 0.6- MW = 897.92 g/mole 0.5- но 0.4- 0.3- ENO 0.2 0.1 0. 400 450 500 550 600 650 700 Absorbance

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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A student mixed 5.00mL of 0.0020M Fe(NO3)3, 3.00mL of 0.0020M KSCN, and 3.00mL of distilled water. The mixture reacted to form a reddish solution indicating that FeSCN2+ was formed, according to the equation below. An absorbance of 0.21 was measured for this solution in a Spectrophotometer set at a wavelength of 447nm. Fe³+ (aq) + SCN (aq) FeSCN2+ (aq) A calibration curve was used to find the equilibrium concentration of the product of this reaction: y = 1895x + 0.027 Calculate the equilibrium concentration of the Fe³+ Report your answer to 4 sig figs
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The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. m = b = RNA (µg) 0.00 10.67 21.34 32.01 42.68 mass= Absorbance at 260 nm 0.224 0.512 0.769 1.145 1.465 Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.636. µg µg
What absorbance corresponds to 1% transmittance? To 50% transmittance?
The % transmittance of a sample was 65.8 %. Determine the absorbance this sample? 0.274 0.773 0.382 0.182 O 1.82
A calibration curve is made for the absorbance as a function of concentration of blue dye (in mg/L). The slope of the curve is 0.131; the y-intercept is -0.01869; the correlation is 0.993. If a consumer sample has an absorbance of 0.120 on the same instrument, what is its concentration of blue dye, in mg/L? a. 0.034 b. 0.935 c. 0.993 d. 1.06
4 of 15 Abs 2.0 1.5 1.0 0.5 0.25 0. 0.2 0.4 0.6 Concentration (M) With reference to the UV-Vis calibration curve reported above, calculate the concentration (Molar) of a sample whose Absorbance is 1.0. Report your answer with two significant figures.
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