The diagram below represents the tryptophan operon with the trp leader MRNA transcript eniarged to represent the AUG translation start codon, two consecutive tryptophan amino acid codons (UGGUGG), and 4 regions (1, 2, 3, and 4) that base pair to form different hairpin-loop structures in the MRNA leader region. Suppose a mutant bacteria has region 4 of the trp operon attenuator region mutated so that it cannot base pair normally. Would the bacteria grow in the absence of the amino acid tryptophan? (hint: in order for bacteria to grow in absence of tryptophan it should be able to synthesize its own tryptophan) Lead&r region trpE trpD trpC trpB trpA DNA 5' 3' Transcription trp leader sequence mRNA AUG UGGUGG (tryptophan codons) 2 UUUUUU O No O There is insutficient information to answer the question. O Yes

Q: When researchers analyzed the gad operon they found the following: Expression of the gad operon at…

A: Here we saw that mutant 1 is causing the expression of gad protein even at pH7 which was not…

Q: In the lac operon system, do the inducers act at the transcription or translation level? Explain why…

A: The lactose operon (lac operon) consists of a regulatory gene (i-gene) and three structural genes -…

Q: 1) The logic of the Lac operon was deciphered by using bacterial lac-operon mutants. Crucial to the…

A: Lac operon is a genetic unit in bacteria that helps in the metabolism of lactose in E.coli and lac…

Q: The TRP operon functions by attenuation. Briefly explain how attenuation works and why it is…

A: Trp operon or the tryptophan operon is a group of genes that are responsible for tryptophan enzymes.…

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Q: Propose a final method of gene regulation of the Up Lateoperon using an updated drawn figure of the…

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Q: It has been postulated that thiogalactoside transacetylase (LacA in the lactose operon) plays a role…

A: Introduction  An operon is a DNA functional unit that comprises a cluster of genes regulated by a…

Q: Considering the Lac operon in E. coli, and chose what the expression of Lac Y would be in:  I) E.…

A: Lac l is the gene  which synthesizes repressor.Actually repressor is a protein that binds to the…

Q: Consider the lac operon of E. coli. Specifically, explain the following: a. Describe the overall…

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Q: Consider a mutant of E. coli that has an inactivating mutation in the gene for adenylate cyclase…

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Q: that best fits the phrase below (1-20) Cis-acting B Post-transcriptional modification…

A: Francis Crick proposed central dogma. The RNA is formed from DNA with the help of enzyme RNA…

Q: The arabinose operon How does the araC protein regulate of the arabinose operon. How does its…

A: How arac protein regulate arabinose operon and how binding differ  If arabinose is absent, the dimer…

Q: Predict the growth/color pattern you'd expect for constitutive mutants for the lac operon. Table 1:…

A:

Q: Describe the main functional differences between the TRP and the Lac operons. Main functional…

A: TRP stands for tryptophan. It is an essential amino acid that must be obtained from the diet and is…

Q: Match each of the following mechanisms with how it can change the levels of gene products--whether…

A: Operons are DNA segments that include groups of associated genes. They consist of a promoter region,…

Q: 12) The top operon in E. coli is a group of genes that control the biosynthesis of tryptophan.…

A: Step 1: TRP operon in E. coli is a classical case of gene regulation in prokaryotes' It regulates…

Q: 1) The UR operon which is composed of sequences A, B, C, and D (which may or may not be genes of…

A: An operon may be considered as a group of certain genes which function together so as to regulate…

Q: If the levels of both Glucose and Lactose were low in a bacterial cell, describe the state of the…

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Q: Mutation in the regulatory gene in the lac operon results in the formation of an inactive repressor.…

A: The lac operon is an operon, or group of genes with a single promoter.The genes in the operon encode…

Q: You have created a null mutation of tRNA synthase for trp-tRNA (the synthase enzyme attaches the trp…

A: A null mutation is a mutation that completely removes a gene or part of a gene.In this case, the…

Q: 15a) Most amino acid biosynthetic operons in prokaryotes have a structure similar to that observed…

A: The tryptophan operon consists of five structural genes: trpE, trpD, trpC, trpB, trpA that codes for…

Q: Bacteria with a lac phenotype are unable to use lactose because of some defect in expressing the…

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Q: ac operon.  Drag the listed molecules to their corresponding bin. Reset Help   ONPG IPTG X-Gal…

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Q: Given the following mutant and conditions, predict the expression of the lacZ gene (no expression,…

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Q: Strain P77 has a mutation in the lacO that prevents it from being bound by the lacI protein.…

A: The operon model is applicable in bacterial gene regulation and also applicable in some lower…

Q: Suppose you have an E. coli in which the gene that codes for the lac repressor protein has been…

A: Lac operon is an inducible type of operon that involves the breakdown of lactose in E.coli.  The lac…

Q: For the wildtype E. coli two sugars influence the level of transcription of the lactose operon. Fill…

A: Sugars in the growth media cAMP levels (High or Low) Positive regulation occurs (Yes…

Q: Regarding the tryptophan operon in bacteria, in the presence of tryptophan, tryptophan binds to the

A: Q. Regarding the tryptophan operon in bacteria, in the presence of tryptophan, tryptophan binds to…

Q: in the lac operon, both the operator (o1) and the binding site for CRP–cAMP show rotational…

A: Lactose operon is also called as lac operon. The operon required for the transport and metabolism of…

Q: Predict a possible phenotype from the following mutations in the Lac Operon: a deletion in…

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Q: Given the Table below concerning the lac operon, match the letter with what would be occurring for…

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Q: 9. Give the levels of Beta-galactosidase activity (high or low or zero) expected for the following…

A: A set of genes present in E.coli. that regulates the uptake of lactose from the surrounding…

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.