The concentration of caffeine in an energy soft drink was determined by standard addition using HPLC. The following procedure was used: a standard caffeine solution was prepared by adding 0.09850g of caffeine to 25.0 cm3 of water.A series of solutions were prepared by adding known volumes of the standard solution to the soft drink before diluting to 100 cm3. The volume of standard and the caffeine peak area are shown in the table: Energy Drink (cm3) Added caffeine Std (cm3) Peak area       15 0 965   15 5 1352   15 10 1739   15 15 2126   15 20 25

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The concentration of caffeine in an energy soft drink was determined by standard addition using HPLC. The following procedure was used: a standard caffeine solution was prepared by adding 0.09850g of caffeine to 25.0 cm3 of water.A series of solutions were prepared by adding known volumes of the standard solution to the soft drink before diluting to 100 cm3. The volume of standard and the caffeine peak area are shown in the table:

Energy Drink (cm3)

Added caffeine Std (cm3)

Peak area

 
 
 

15

0

965

 

15

5

1352

 

15

10

1739

 

15

15

2126

 

15

20

2513

 

 

 

Determine the concentration of caffeine in the soft drink as mg cm-3.

 
  • A.

    3.3

  • B.

    2.3

  • C.

    4.3

  • D.

    7.3

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