The breaking buffer that we use this week contains 10mM Tris, pH 8.0, 150mM NaCl. The elution buffer is breaking buffer that also contains 300mM imidazole. The instructor made 3L of elution buffer for all the students. The instructor had 500ml of 4M of Tris (121.1 g/mole) (pH8.0), 750ml of 5M NaCl (MW = 58.44 g/mole) and1.5Ll of 1M imidazole (68.08 g/mole). How much of each stock solution did they need? Show your calculations for credit.
The breaking buffer that we use this week contains 10mM Tris, pH 8.0, 150mM NaCl. The elution buffer is breaking buffer that also contains 300mM imidazole. The instructor made 3L of elution buffer for all the students. The instructor had 500ml of 4M of Tris (121.1 g/mole) (pH8.0), 750ml of 5M NaCl (MW = 58.44 g/mole) and1.5Ll of 1M imidazole (68.08 g/mole). How much of each stock solution did they need? Show your calculations for credit.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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6 The breaking buffer that we use this week contains 10mM Tris, pH 8.0, 150mM NaCl. The elution buffer is breaking buffer that also contains 300mM imidazole. The instructor made 3L of elution buffer for all the students. The instructor had 500ml of 4M of Tris (121.1 g/mole) (pH8.0), 750ml of 5M NaCl (MW = 58.44 g/mole) and1.5Ll of 1M imidazole (68.08 g/mole). How much of each stock solution did they need? Show your calculations for credit.
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