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The Bradford protein assay is a dye-binding assay based on the differential color change of a dye in response to various concentrations of protein. It is used to measure the total protein concentration in a sample. This assay makes use of a Bovine Serum Albumin (BSA) as a standard which is a globular protein. This is done by comparing an unknown quantity of protein to known amounts of the protein standard being used. The protein standard is measured, and its absorbance and concentration are plotted on a graph to create the standard curve. This curve is then used to determine the concentration of the unknown protein. The table below presents the standard calibration of the BSA standard for a study on freshly isolated brain microvessel of Sprague-Dawley male and female rats at a wavelength of 595 nm and pathlength of 1-cm at room 25°C. BSA Concentration (mg/mL) Absorbance 0 0.125 0.25 0.50 0.75 1.0 1.5 20 Determine the protein concentration in the brain microvessel of a female Sprague-Dawley rat if the absorbance reading of the sample is 0.315. 0 0.06 0.14 0.25 0.36 0.52 0.67 0.68 https://www.researchgate.net/publication/325018386 O 0.196 g/L 0.939 mg/L 0.096 mg/L 0.366 mg/L 0 0714 mp/l

The Bradford protein assay is a dye-binding assay based on the differential color change of a dye in response to various concentrations of protein. It is used to measure the total protein concentration in a sample. This assay makes use of a Bovine Serum Albumin (BSA) as a standard which is a globular protein. This is done by comparing an unknown quantity of protein to known amounts of the protein standard being used. The protein standard is measured, and its absorbance and concentration are plotted on a graph to create the standard curve. This curve is then used to determine the concentration of the unknown protein. The table below presents the standard calibration of the BSA standard for a study on freshly isolated brain microvessel of Sprague-Dawley male and female rats at a wavelength of 595 nm and pathlength of 1-cm at room 25°C. BSA Concentration (mg/mL) Absorbance 0 0.125 0.25 0.50 0.75 1.0 1.5 20 Determine the protein concentration in the brain microvessel of a female Sprague-Dawley rat if the absorbance reading of the sample is 0.315. 0 0.06 0.14 0.25 0.36 0.52 0.67 0.68 https://www.researchgate.net/publication/325018386 O 0.196 g/L 0.939 mg/L 0.096 mg/L 0.366 mg/L 0 0714 mp/l

Introduction to Chemical Engineering Thermodynamics
Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Publisher:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
Chapter1: Introduction
Section: Chapter Questions
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The Bradford protein assay is a dye-binding assay based on the differential color change of a dye in response to various concentrations of protein. It is used to measure the total protein concentration in a sample. This assay makes use of a Bovine Serum Albumin (BSA) as a standard which is a globular protein. This is done by comparing an unknown quantity of protein to known amounts of the protein standard being used. The protein standard is measured, and its absorbance and concentration are plotted on a graph to create the standard curve. This curve is then used to determine the concentration of the unknown protein. The table below presents the standard calibration of the BSA standard for a study on freshly isolated brain microvessel of Sprague-Dawley male and female rats at a wavelength of 595 nm and pathlength of 1-cm at room 25°C. BSA Concentration (mg/mL) Absorbance 0 0.125 0.25 0.50 0.75 1.0 1.5 2.0 0 0.06 0.14 0.25 0.36 0.52 0.67 0.68 Determine the protein concentration in the brain microvessel of a female Sprague-Dawley rat if the absorbance reading of the sample is 0.315. https://www.researchgate.net/publication/325018386 O 0.196 g/L O 0.939 mg/L o 0.096 mg/L O 0.366 mg/l 0 0.714 mg/L
Transcribed Image Text:The Bradford protein assay is a dye-binding assay based on the differential color change of a dye in response to various concentrations of protein. It is used to measure the total protein concentration in a sample. This assay makes use of a Bovine Serum Albumin (BSA) as a standard which is a globular protein. This is done by comparing an unknown quantity of protein to known amounts of the protein standard being used. The protein standard is measured, and its absorbance and concentration are plotted on a graph to create the standard curve. This curve is then used to determine the concentration of the unknown protein. The table below presents the standard calibration of the BSA standard for a study on freshly isolated brain microvessel of Sprague-Dawley male and female rats at a wavelength of 595 nm and pathlength of 1-cm at room 25°C. BSA Concentration (mg/mL) Absorbance 0 0.125 0.25 0.50 0.75 1.0 1.5 2.0 0 0.06 0.14 0.25 0.36 0.52 0.67 0.68 Determine the protein concentration in the brain microvessel of a female Sprague-Dawley rat if the absorbance reading of the sample is 0.315. https://www.researchgate.net/publication/325018386 O 0.196 g/L O 0.939 mg/L o 0.096 mg/L O 0.366 mg/l 0 0.714 mg/L
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