The activity of chymotrypsin changes as the pH changes in the range of pH 5-9, as shown on the a. What amino acid functional group on which residue following graph. From your understanding of the chemical mechanism of the chymotrypsin. reaction, explain the pH effect on chymotrypsin activity by answering parts a, b, and c. is most likely responsible for the effect of PH on catalysis? Percent activity (%) 100 60 50 40 20 0 5.0 5.5 6.0 6.5 7.0 7.5 8.0 pH 8.5 9.0 b. What is the apparent pKa of the functional group. Indicate on the graph how you would determine the pka. c. Briefly explain in terms of the chymotrypsin mechanism why the activity of the enzyme increases as the pH increases in this range.

The activity of chymotrypsin changes as the pH changes in the range of pH 5-9, as shown on the a. What amino acid functional group on which residue following graph. From your understanding of the chemical mechanism of the chymotrypsin. reaction, explain the pH effect on chymotrypsin activity by answering parts a, b, and c. is most likely responsible for the effect of PH on catalysis? Percent activity (%) 100 60 50 40 20 0 5.0 5.5 6.0 6.5 7.0 7.5 8.0 pH 8.5 9.0 b. What is the apparent pKa of the functional group. Indicate on the graph how you would determine the pka. c. Briefly explain in terms of the chymotrypsin mechanism why the activity of the enzyme increases as the pH increases in this range.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

Please Explain each part to the best of your ability. This is Biochemistry. Thank you

1.
The activity of chymotrypsin changes as the pH changes in the range of pH 5-9, as shown on the a. What amino acid functional group on which residue
following graph. From your understanding of the chemical mechanism of the chymotrypsin
reaction, explain the pH effect on chymotrypsin activity by answering parts a, b, and c.
is most likely responsible for the effect of PH on
catalysis?
Percent activity (%)
100
80
60
50
40
20
0
5.0
5.5
6.0
6.5
7.0
pH
1
1
7.5 8.0 8.5 9.0
b. What is the apparent pKa of the functional group.
Indicate on the graph how you would determine the
pka.
c. Briefly explain in terms of the chymotrypsin
mechanism why the activity of the enzyme increases
as the pH increases in this range.

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