Tetracycline produced in Streptomyces aureus fermentations is purified by crystallisation. One hundred kg of a supersaturated solution containing 7.7 wt% tetracycline is cooled in a batch fluidised-bed crystalliser. Seed crystals of tetracycline are added at a concentration of 40 ppm to promote crystal growth. At the end of the crystallisation process, the remaining solution contains 2.8% tetracycline. (a) What is the mass of the residual tetracycline solution? (b) What mass of tetracycline crystals is produced?
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- Human saliva contains the enzyme α-amylase which hydrolyzes polysaccharide acetal linkages. A soda cracker (containing starch) was carefully ground; the crumbs were distributed evenly between two test tubes. The first test tube contained only water, while the second contained a 1% solution of α-amylase. Both mixtures were heated to 35°C for 10 minutes and filtered. The filtrates were tested with iodine solution and Benedict’s solution. Use the following results to answer the questions below. Mixture Iodine test Benedict’s Test cracker in water dark blue blue cracker in water and α-amylase red red-orange precipitate a. Was the starch from the cracker/water mixture hydrolyzed? Was the starch from the cracker in the 1% α-amylase solution hydrolyzed? b. Which cracker contained reducing sugars?20 Carbamazepine tablets were found to weigh 10.000 g in total. The tablets were ground to a fine powder using a pestle and mortar. A 313.2 mg sample of the powder was boiled with 25ml ethanol for a few minutes. The hot mixture was stirred in a closed flask for 10 minutes and filtered through sintered glass. The flask and filter were washed with ethanol and the washings combined with the filtrate. The cooled mixture was made up to 100ml with ethanol (soln A). 5ml of the ethanol extract was diluted to 250ml with ethanol (soln B) and the absorbance of the resulting solution was found to be 0.598 absorbance units at a wavelength of 285nm. The A1%,1cm was 490? a) Calculate the total amount (in mg) of carbamazepine in solution B (1 decimal) Answer = mg b) Calculate the total amount (in mg) of carbamazepine in solution A (1 decimal) Answer = mg c) Calculate the amount (in mg) of carbamazepine in a single tablet. (1 decimal) Answer =You have an aqueous solution of pyridinium chloride (C6H5N'HCI) and you want to extract it with a second solvent where it would be more soluble. Which of the following should you do to accomplish the said task? Mix 3M NaOH, acetone, and the aqueous layer in a separatory funnel, shake, release build-up pressure, return to A stand, and let the mixture separate into two layers. Mix methanol and the aqueous layer in a separatory funnel, shake, release build-up pressure, return to stand, and let the mixture separate into two layers. Mix 3M NaOH, toluene, and the aqueous layer in a separatory funnel, shake, release build-up pressure, return to stand, and let the mixture separate into two layers. Mix dichloromethane and the aqueous layer in a separatory funnel, shake, release build-up pressure, return to stand, D and let the mixture separate into two layers.
- A 500.0 μL aliquot of a ferric chloride solution is added to a 50.00 mL volumetric flask. To this flask is added 10 mL of 2M sodium acetate, 2 mL of 10 wt% NH4OCI, 3 mL of 5 wt% 1,10-phenanthroline, and enough DI water to fill the remaining volume of the flask for a total volume of 50.00 mL. Based on the UV-Visible light absorbance, the concentration of [Fe2+]=2.6 ppm. What is the concentration of Fe3+ ion in the original ferric chloride solution? O 3.85 X 103 ppm O 260.0 ppm O 260 ppm O 130 ppmThe aqueous solution of acetic with a flow rate of 1000 kg/hr contains 30% acetic acid by mass and will be extracted in a countercurrent multistage process with pure isopropyl ether (IPE) to reduce the acetic acid concentration in the final raffinate phase (LN) to 2% by mass. a) Calculate the minimum solvent flow. b) Determine the theoretical number of steps required when using a solvent of 1.5 minimum solvent flow rate. c) Determine the number of theoretical steps using the McCabe-Thiele method. Chart. Acetic acid (A)-Water (B)-isopropyl ether (IPE) (C) LIQUID-Liquid Balances at 1 atm pressure and 293 K Water layer (%) Water (B) Isopropyl ether layer (%) Water (B) 0.6 Acetic acid (A) IPE (C) Acetic acid (A) IPE (C) 98.8 1.2 99.4 99.3 98.9 0.69 98.1 1.2 0.18 0.5 1.41 97.1 1.5 0.37 0.7 2.89 95.5 1.6 0.79 98.4 91.7 1.9 1.0 97.1 6.42 13.30 25.50 36.70 1.9 93.3 4.82 11.40 2.3 84.4 71.1 3.4 3.9 84.7 71.5 21.60 31.10 58.9 4.4 6.9 58.1 48.7 10.6 10.8 45.1 37.1 44.30 46.40 16.5 36.20 15.1…1. In a process of concentrating 1000 kg/hr of freshly extracted juice (orange) containing 12.5% solids, the juice is strained yielding 800 kg/hr of strained juice and 200 kg of pulpy juice. The strained juice is concentrated in an evaporator to give an evaporated juice of 58% solids. The pulpy juice is bypassed around the evaporator and mixed with the evaporated juice in a mixer to improve the flavor. This final concentrated juice is 42% solids. Calculate a) the solids concentration in the strained juice b.) the kg/hr of final concentrated juice and c) the concentration of solids in the pulpy juice bypassed.
- 1000 kg of a solution of citric acid (C6H8O7) and water contains 5% acid and 95 % water. Citric Acid is removed from water using benzene(C6H6) as the extraction solvent. Benzene and water are immiscible. When an aqueous solution of acid is thoroughly mixed with pure benzene and allowed to settle, two layers of liquid are formed. The benzene layer contains 90% benzene and 10% citric acid; the water layer contains 1 % benzene, 0.5% acid and the rest water. a. Calculate the weight of the benzene used in this operation. b. Calculate the weight of the benzene layer.For a 22.16 mg sample of MgC2O4•H2O (Mwt = 130.35 g/mol) shows two steps:a loss of 3.06 mg from 100–250 oC and a loss of 12.24 mg from 350–550oC. For each step, identify the volatilization product and the solidresidue that remains. In second step, two volatile products are formed.(Mwt C = 12 g/mol; O = 16 g/mol; H = 1 g/mol)1. Calculate the amount of 8% cut back orange juice that is required to produce 1000 kg orange concentrate at 45%. Also calculate the water lost. The concentrate is prepared by first evaporating 8% straight juice to 65% concentrate before dilution with cut-back juice for flavouring purposes.
- Calculate the mass flow rates of 15% and 60% by mass sucrose solution which must be mixed to prepare 100 kg/h of 30% sucrose solution. Mass flow rate of 15% solution: Mass flow rate of 60% solution:The solubility of suberic acid C6H12(COOH)2 is 0.14 g/100 ml H2O and 0.56g/100 ml ethyl ether at 15 0C. Calculate the weight of suberic acid removed from 50 ml aqueous solution containing 40 mg acid by extraction with 50 ml ether. Calculate the weight of acid that would be removed by two successive 25 ml extraction with ether.An experiment was done : Synthesis of a potential enzyme inhibitor The product achieved from the experiment was 4.0g. Using the picture provided determine the percentage yield.