Table 5: Effect of Temperature on Sucrase Activity Optical Density Temperature 10 °C (50 °F) 20 °C (68 "F) 30 °C (86 "F) 40 °C (104 °F) 50 °C (122 "F) 60 °C (140 F) 70 °C (158 °F) 0.005 0.275 0.772 0.934 0.927 0.828 0.606 2 0.007 0.285 0.791 0.956 0.934 0.843 0.634 0.007 0.260 0.785 0.963 0.923 0.837 0.600 0.273 0.783 0.951 0.928 0.836 0.613 Average 0.006 Effect of Temperature on Sucrase Activity Glucose (O.D. units) 0.8 0.6 + 0.4 + 0.2 10 20 30 40 50 60 70 3. State the optimum temperature for sucrase activity and describe how sucrase activity changes at lower and higher temperatures. 4. Was sucrase activity higher at 25 °C or 55 °C?

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