Table 4: Effect of pH on Sucrase Activity Optical Density pH 3 pH 4 pH 5 pH 6 pH 7 pH 8 pH 9 0.157 0.324 0.659 0.937 0.796 0.426 0.147 0.146 0.327 0.632 0.940 0.774 0.408 0.157 3 0.151 0.307 0.637 0.949 0.824 0.434 0.156 Average 0.151 0.319 0.643 0.942 0.798 0.423 0.153 Effect of pH on Sucrase Activity Glucose (O.D. units) 0.8 0.6 0.4 + 0.2 pH 2 4 6 8 10 1. State the optimum pH for sucrase activity and describe how sucrase activity changes at more acidic and more alkaline pH values. 2. Was the rate of increase of sucrase activity higher at a pH of 8.5 or a pH of 5.5?

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