Suppose a researcher has three different Drosophila strains that have mutations in the bicoid gene called bicoid-A, bicoid-B, and bicoid-C; the wild type is designated bicoid. To study these mutations, phenotypically normal female flies that are homozygous for the given bicoid mutation were obtained, and their oocytes were analyzed using a Northern blot to determine the size and/or amount of the bicoid mRNA and in situ hybridization to determine the bicoid mRNA location within the oocyte. A wild-type strain was also analyzed as a control. In both cases, the probe was complementary to the bicoid mRNA and the results are shown below. (Anterior is on the left; posterior is on the right.) Northern blot 1 2 3 Wild type 4 bicoid-B Lane 1. Wild type (bicoid*) Lane 2. bicoid-A In situ hybridization Lane 3. bicoid-B Lane 4. bicoid-C bicoid-A bicoid-C Which mutation is likely to cause the embryo to develop two "anterior" ends? bicoid-B bicoid-A bicoid-C

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Suppose a researcher has three different Drosophila strains that have mutations in the bicoid gene called bicoid-A, bicoid-B, and bicoid-C; the wild type is designated bicoid +. To study these mutations,
phenotypically normal female flies that are homozygous for the given bicoid mutation were obtained, and their oocytes were analyzed using a Northern blot to determine the size and/or amount of the bicoid
mRNA and in situ hybridization to determine the bicoid mRNA location within the oocyte. A wild-type strain was also analyzed as a control. In both cases, the probe was complementary to the bicoid mRNA and
the results are shown below. (Anterior is on the left; posterior is on the right.)
Northern blot
1
2
-
3 4
In situ hybridization
Wild type
Lane 1. Wild type (bicoid*)
Lane 2. bicoid-A
Lane 3. bicoid-B
Lane 4. bicoid-C
bicoid-B
bicoid-A
bicoid-C
Which mutation is likely to cause the embryo to develop two "anterior" ends?
bicoid-B
Obicoid-A
bicoid-C
Transcribed Image Text:Suppose a researcher has three different Drosophila strains that have mutations in the bicoid gene called bicoid-A, bicoid-B, and bicoid-C; the wild type is designated bicoid +. To study these mutations, phenotypically normal female flies that are homozygous for the given bicoid mutation were obtained, and their oocytes were analyzed using a Northern blot to determine the size and/or amount of the bicoid mRNA and in situ hybridization to determine the bicoid mRNA location within the oocyte. A wild-type strain was also analyzed as a control. In both cases, the probe was complementary to the bicoid mRNA and the results are shown below. (Anterior is on the left; posterior is on the right.) Northern blot 1 2 - 3 4 In situ hybridization Wild type Lane 1. Wild type (bicoid*) Lane 2. bicoid-A Lane 3. bicoid-B Lane 4. bicoid-C bicoid-B bicoid-A bicoid-C Which mutation is likely to cause the embryo to develop two "anterior" ends? bicoid-B Obicoid-A bicoid-C
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