Standard Tubes Volume of 0.1 Hydroxamate standard Tubes Optical Density (OD) Concentration 0.061 0.078 0.095 1 0.1 0.2 0.4 0.002 3 4 0.004 0.125 0.008 Standard Curve of Optical Density (OD) vs Concentration of Hydroxamate 0.14 0.12 0.1 520 nm

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
icon
Related questions
Question

Write a conclusion based on the result above

Standard Tubes
Optical Density
(OD)
Tubes
Volume of 0.1
Concentration
Hydroxamate
standard
1
0.061
0.1
0.078
0.002
3
0.2
0.095
0.004
4
0.4
0.125
0.008
Standard Curve of Optical Density (OD) vs Concentration of
Hydroxamate
0.14
0.12
0.1
0.08
ý = 7.9857x + 0.0618
R = 0.9988
0.06
0.04
0.02
0.001
0.002
0.003
0.004
0.005
0.006
0.007
0.008
0.009
Concentration of Hydroxamate
Figure 1: Graph of Standard Curve of Optical Density against the Concentration of
Hydroxamate.
Hydroxamate Assay Tubes
From Fig 1, the equation displayed was y = 7.9857x + 0.0618 and the R2-0.9988. From the
equation, y intercept was the optical density and x-intercept was the concentration of
hydroxamate.
Tubes
Optical Density at 520 nm
Concentration of
0.103
0.077
0.123
hydroxamate formed
0.0052
0.0019
0.0077
0.0092
6
7
0.135
0.114
0.0065
10
0.065
0.0004
Optical Density at 520 nm
Transcribed Image Text:Standard Tubes Optical Density (OD) Tubes Volume of 0.1 Concentration Hydroxamate standard 1 0.061 0.1 0.078 0.002 3 0.2 0.095 0.004 4 0.4 0.125 0.008 Standard Curve of Optical Density (OD) vs Concentration of Hydroxamate 0.14 0.12 0.1 0.08 ý = 7.9857x + 0.0618 R = 0.9988 0.06 0.04 0.02 0.001 0.002 0.003 0.004 0.005 0.006 0.007 0.008 0.009 Concentration of Hydroxamate Figure 1: Graph of Standard Curve of Optical Density against the Concentration of Hydroxamate. Hydroxamate Assay Tubes From Fig 1, the equation displayed was y = 7.9857x + 0.0618 and the R2-0.9988. From the equation, y intercept was the optical density and x-intercept was the concentration of hydroxamate. Tubes Optical Density at 520 nm Concentration of 0.103 0.077 0.123 hydroxamate formed 0.0052 0.0019 0.0077 0.0092 6 7 0.135 0.114 0.0065 10 0.065 0.0004 Optical Density at 520 nm
Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Activities
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.
Similar questions
Recommended textbooks for you
Chemistry
Chemistry
Chemistry
ISBN:
9781305957404
Author:
Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:
Cengage Learning
Chemistry
Chemistry
Chemistry
ISBN:
9781259911156
Author:
Raymond Chang Dr., Jason Overby Professor
Publisher:
McGraw-Hill Education
Principles of Instrumental Analysis
Principles of Instrumental Analysis
Chemistry
ISBN:
9781305577213
Author:
Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:
Cengage Learning
Organic Chemistry
Organic Chemistry
Chemistry
ISBN:
9780078021558
Author:
Janice Gorzynski Smith Dr.
Publisher:
McGraw-Hill Education
Chemistry: Principles and Reactions
Chemistry: Principles and Reactions
Chemistry
ISBN:
9781305079373
Author:
William L. Masterton, Cecile N. Hurley
Publisher:
Cengage Learning
Elementary Principles of Chemical Processes, Bind…
Elementary Principles of Chemical Processes, Bind…
Chemistry
ISBN:
9781118431221
Author:
Richard M. Felder, Ronald W. Rousseau, Lisa G. Bullard
Publisher:
WILEY