Standard Tubes Volume of 0.1 Hydroxamate standard Tubes Optical Density (OD) Concentration 0.061 0.078 0.095 1 0.1 0.2 0.4 0.002 3 4 0.004 0.125 0.008 Standard Curve of Optical Density (OD) vs Concentration of Hydroxamate 0.14 0.12 0.1 520 nm
Standard Tubes Volume of 0.1 Hydroxamate standard Tubes Optical Density (OD) Concentration 0.061 0.078 0.095 1 0.1 0.2 0.4 0.002 3 4 0.004 0.125 0.008 Standard Curve of Optical Density (OD) vs Concentration of Hydroxamate 0.14 0.12 0.1 520 nm
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Write a conclusion based on the result above
![Standard Tubes
Optical Density
(OD)
Tubes
Volume of 0.1
Concentration
Hydroxamate
standard
1
0.061
0.1
0.078
0.002
3
0.2
0.095
0.004
4
0.4
0.125
0.008
Standard Curve of Optical Density (OD) vs Concentration of
Hydroxamate
0.14
0.12
0.1
0.08
ý = 7.9857x + 0.0618
R = 0.9988
0.06
0.04
0.02
0.001
0.002
0.003
0.004
0.005
0.006
0.007
0.008
0.009
Concentration of Hydroxamate
Figure 1: Graph of Standard Curve of Optical Density against the Concentration of
Hydroxamate.
Hydroxamate Assay Tubes
From Fig 1, the equation displayed was y = 7.9857x + 0.0618 and the R2-0.9988. From the
equation, y intercept was the optical density and x-intercept was the concentration of
hydroxamate.
Tubes
Optical Density at 520 nm
Concentration of
0.103
0.077
0.123
hydroxamate formed
0.0052
0.0019
0.0077
0.0092
6
7
0.135
0.114
0.0065
10
0.065
0.0004
Optical Density at 520 nm](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa7555c48-a334-459a-ae1f-ef0a9e775694%2F752795db-ad6f-4227-9b17-417978d67857%2Fghjotj6_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Standard Tubes
Optical Density
(OD)
Tubes
Volume of 0.1
Concentration
Hydroxamate
standard
1
0.061
0.1
0.078
0.002
3
0.2
0.095
0.004
4
0.4
0.125
0.008
Standard Curve of Optical Density (OD) vs Concentration of
Hydroxamate
0.14
0.12
0.1
0.08
ý = 7.9857x + 0.0618
R = 0.9988
0.06
0.04
0.02
0.001
0.002
0.003
0.004
0.005
0.006
0.007
0.008
0.009
Concentration of Hydroxamate
Figure 1: Graph of Standard Curve of Optical Density against the Concentration of
Hydroxamate.
Hydroxamate Assay Tubes
From Fig 1, the equation displayed was y = 7.9857x + 0.0618 and the R2-0.9988. From the
equation, y intercept was the optical density and x-intercept was the concentration of
hydroxamate.
Tubes
Optical Density at 520 nm
Concentration of
0.103
0.077
0.123
hydroxamate formed
0.0052
0.0019
0.0077
0.0092
6
7
0.135
0.114
0.0065
10
0.065
0.0004
Optical Density at 520 nm
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