For the following questions (1-5), consider the compound tartrazine (Yellow 5 food dye) that has a chemical formula of C16H9N4Na3O9S2. Ana weighs out 0.1328 g of tartrazine powder on the balance for her experiment and makes a stock solution by dissolving her powder in 500.0 mL of solution.
1. Determine the number of moles of tartrazine that Ana weighed out.
2. Find the molarity (mol/L) of Ana’s tartrazine solution.
3. Ana makes 5 tartrazine standards by diluting her solution and measures their absorbances using a spectrophotometer:
4. Calculate the molar extinction coefficient (molar absorptivity) of tartrazine in units of 1/(M·cm). Assume a cuvette path length of 1.00 cm.
5. If Ana determines the absorbance of an unknown tartrazine sample to be 0.662, what is the concentration of the sample in µM?
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