a. Separation of PeaksBelow are two chromatograms after LC separation. Which chromatogram is better to proceed for MSanalysis? What mobile phase condition/composition (LC separation) was used?HPLC: 15% H₂O, 85% CHCN, CIS Revane Phase, 100 mm long, 5pm particles(1)Area (1)-14750Arta (2)-53538MINHPLC: 59% 10,49% CHCN, CIS Reverse Phase, 100 m long, 5 pm particles(2)Mes (1)-2256A(2)-9536A(3)-2563813H

LCMS is Liquid chromatography–mass spectrometry which is used for mass analysis of unknown or known…

Answered: Separation of Peaks Below are two…

Separation of Peaks Below are two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What mobile phase condition/composition (LC separation) was used?

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

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Question

a. Separation of Peaks
Below are two chromatograms after LC separation. Which chromatogram is better to proceed for MS
analysis? What mobile phase condition/composition (LC separation) was used?
HPLC: 15% H₂O, 85% CHCN, CIS Revane Phase, 100 mm long, 5pm particles
(1)
Area (1)-14750
Arta (2)-53538
MIN
HPLC: 59% 10,49% CHCN, CIS Reverse Phase, 100 m long, 5 pm particles
(2)
Mes (1)-2256
A(2)-9536
A(3)-25638
13H

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