Select all that apply to a direct simple stain: O uses only 1 dye in the procedure O uses multiple dyes in the procedure can be used to determine cell morphology, size, and arrangement. O does not involve fixing does involve fixing does not involve a washing step does involve a washing step O the dye is basic or positively charged and stains the bacteria O the dye is acidic or negatively charged and stains the background Ocells may be distorted or shrunken cells will never be distorted or shrunken
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- Select all that apply to a negative stain: uses only 1 dye in the procedure O uses multiple dyes in the procedure involves fixing does not involve fixing O involves a washing step does not involve a washing step uses a basic or positively charged dye which stains the bacterial cells uses an acidic or negatively charged dye which stains the background O can show cell morphology, size, and arrangement Microsoft Bing 11:33 AM 87°F Sunny 9/14/2021Select all that apply to a direct simple stain: 1. the dye is acidic or negatively charged and stains the background 2. uses multiple dyes in the procedure 3. cells will never be distorted or shrunken 4. uses only 1 dye in the procedure 5. does involve a washing step 6. can be used to determine cell morphology, size, and arrangement. 7. does involve fixing 8. the dye is basic or positively charged and stains the bacteria 9. cells may be distorted or shrunken 10. does not involve a washing step 11. does not involve fixingThe benefits of the negative stain include: Mark all that apply: O No shrinkage or distortion of cells due to no heat or methanol fixing Because the cells don't take up stain they don't get shrunk or distorted O One can more accurately determine cell size and shape because the cells stand out against the background One can more accurately determine the cell size and shape because there is no shrinkage or distortion of cells O Negatively charged dyes are safer to work with than positively charged dyes IMicrosoft B 11:32 A
- Which of the following statements is FALSE? Question options: stains increase the contrast of a specimen with its surroundings all of these statements are TRUE dyes generally consist of a chromophore and an ion the chromophore of an acidic dye is positively-charged simple staining procedures use a single dye basic dyes bind to negatively-charged moleculesWhat is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide? Paragraph BIUSelect all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted or shrunken 3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cells
- How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: O Water must be added to a smear from liquid media but not from solid O Water must be added to a smear made from solid media but not liquid media A smear made from liquid media will be more thick than a smear made from solid O A smear made from liquid media will be thinner than a smear made from solid media O A smear made from liquid media doesn't have to be fixed but a smear from solid media does Microsoft Bing 11:31 AM 87°F Sunny 9/14/2021Is acid fast staining differential stain , if yes, what are the 2 types of stain usedThe benefits of the negative stain include: Mark all that apply: 1. No shrinkage or distortion of cells due to no heat fixing 2. Because the cells don't take up stain they don't get shrunk or distorted 3. One can more accurately determine the cell size and shape because there is no shrinkage or distortion of cells 4. Negatively charged dyes are safer to work with than positively charged dyes 5. One can more accurately determine cell size and shape because the cells stand out against the background
- Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: O So that cells are not clumped and don't entrap stain creating erroneus results So that no contaminants are introduced onto the slide by being entrapped in clumps OSo that the cells are spread out enough that cell morphology can be discerned OSo that the cells are spread out enough that the arrangement can be observed O So that there are small groups of cells clumped together to make them visible Microsoft Bing 11:31 AM 87°F Sunny 9/14/2021INSTRUCTION: Answer the question properly Do not copy in Google, plagiarize checker will be used. QUESTION: Discuss the various theories on the Gram stain.Complete for Additional Differential Staining B: Negative staining ~ thin smear C: Endospore Stain