sample question 2. During further studies on transferrin, the protein was treated with dithiothreitol, then incubated with ICH2CO2H. After this trypsin was added and the products were separated using reversed phase liquid chromatography before being analysed by MALDI mass spectrometry using an a-cyano-4-hydroxycinnamic acid matrix. (i) How would you expect the structure of the protein to be modified by each of the reagents dithiothreitol, ICH2CO2H and trypsin? (ii) Identify two features of a-cyano-4-hydroxycinnamic acid that make it a good MALDI matrix.
sample question 2. During further studies on transferrin, the protein was treated with dithiothreitol, then incubated with ICH2CO2H. After this trypsin was added and the products were separated using reversed phase liquid chromatography before being analysed by MALDI mass spectrometry using an a-cyano-4-hydroxycinnamic acid matrix. (i) How would you expect the structure of the protein to be modified by each of the reagents dithiothreitol, ICH2CO2H and trypsin? (ii) Identify two features of a-cyano-4-hydroxycinnamic acid that make it a good MALDI matrix.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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sample question 2. During further studies on transferrin, the protein was treated with dithiothreitol, then incubated with ICH2CO2H. After this trypsin was added and the products were separated using reversed phase liquid chromatography before being analysed by MALDI mass spectrometry using an a-cyano-4-hydroxycinnamic acid matrix.
-
(i) How would you expect the structure of the protein to be modified by each of the
reagents dithiothreitol, ICH2CO2H and trypsin?
-
(ii) Identify two features of a-cyano-4-hydroxycinnamic acid that make it a good MALDI
matrix.
Transcribed Image Text:HS
OH
OH
Dithiothreitol
HO
CN
SH
CO₂H
a-Cyano-4-hydroxycinnamic acid
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