The sample preparation using the EZ:Faast kit involves many steps. Put the following steps in the correct order, first to last. 5 7 4 6 3 2 1 < > > > Extract the derivatised amino acids using a liquid-liquid extraction. The amino acids are in the organic layer. Remove the organic solvent by drying the sample extracts under Nitrogen gas. Derivatise the amino acids using the 'proprietary' derivatising agent. Reconstitute the derivatised amino acids with HPLC mobile phase, prior to assaying them using LC-MS. Elute the amino acids from the SPE media. Use SPE (solid phase extraction) to isolate free amino acids from sample matrix components. Add internal standard.

The sample preparation using the EZ:Faast kit involves many steps. Put the following steps in the correct order, first to last. 5 7 4 6 3 2 1 < > > > Extract the derivatised amino acids using a liquid-liquid extraction. The amino acids are in the organic layer. Remove the organic solvent by drying the sample extracts under Nitrogen gas. Derivatise the amino acids using the 'proprietary' derivatising agent. Reconstitute the derivatised amino acids with HPLC mobile phase, prior to assaying them using LC-MS. Elute the amino acids from the SPE media. Use SPE (solid phase extraction) to isolate free amino acids from sample matrix components. Add internal standard.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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Spectroanalytical Methods

Spectroanalytical methods measure the wavelength and intensity of radiations that are either emitted or absorbed by a molecular or atomic species. These methods are useful for evaluating the electronic or atomic arrangement of the molecules of a given analyte.

Question

The sample preparation using the EZ:Faast kit involves many steps. Put the following
steps in the correct order, first to last.
5
7
4
6
3
2
1
>
Extract the derivatised amino acids using a liquid-liquid extraction. The
amino acids are in the organic layer.
Remove the organic solvent by drying the sample extracts under Nitrogen
gas.
Derivatise the amino acids using the 'proprietary' derivatising agent.
Reconstitute the derivatised amino acids with HPLC mobile phase, prior to
assaying them using LC-MS.
Elute the amino acids from the SPE media.
Use SPE (solid phase extraction) to isolate free amino acids from sample
matrix components.
Add internal standard.

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