RNA DNA Protein at is the purpose for including a blocking step? Blocking prevents further migration of proteins on the membrane Blocking enhances detection of chemiluminescent substrates Blocking prevents non-specific binding of antibodies at is being removed during the multiple washing steps that are part of a western blotting ocol? Antibodies that are not bound to their target Enzymes that are not bound to a secondary antibody Proteins from the original sample that are not bound by an antibody

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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What kind of molecule is being detected in a western blot?
RNA
DNA
Protein
000
What is the purpose for including a blocking step?
Blocking prevents further migration of proteins on the membrane
Blocking enhances detection of chemiluminescent substrates
Blocking prevents non-specific binding of antibodies
ooo
What is being removed during the multiple washing steps that are part of a western blotting
protocol?
000
Antibodies
that are not bound to their target
Enzymes that are not bound to a secondary antibody
Proteins from the original sample that are not bound by an antibody
Transcribed Image Text:What kind of molecule is being detected in a western blot? RNA DNA Protein 000 What is the purpose for including a blocking step? Blocking prevents further migration of proteins on the membrane Blocking enhances detection of chemiluminescent substrates Blocking prevents non-specific binding of antibodies ooo What is being removed during the multiple washing steps that are part of a western blotting protocol? 000 Antibodies that are not bound to their target Enzymes that are not bound to a secondary antibody Proteins from the original sample that are not bound by an antibody
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