Riboflavin (Vitamin B2) is determined in a cereal sample by measuring its fluorescence intensity(형광세기) in 5% acetic acid solution. A calibration curve was prepared by measuring the fluorescence intensities of a series of standards of increasing concentrations. The following data were obtained. Riboflavin (μg/mL) 0.000 0.100 0.200 0.400 0.800 Unknown sample Fluorescence intensity 0.0 5.8 12.2 22.3 43.3 15.4 (a) Use the method of least squares to obtain the best straight line through these five points (n=5). (b) Make a graph showing the experimental data a
Riboflavin (Vitamin B2) is determined in a cereal sample by measuring its fluorescence intensity(형광세기) in 5% acetic acid solution. A calibration curve was prepared by measuring the fluorescence intensities of a series of standards of increasing concentrations. The following data were obtained.
|
Riboflavin (μg/mL) |
0.000 |
0.100 |
0.200 |
0.400 |
0.800 |
Unknown sample |
|
Fluorescence intensity |
0.0 |
5.8 |
12.2 |
22.3 |
43.3 |
15.4 |
(a) Use the method of least squares to obtain the best straight line through these five points (n=5).
(b) Make a graph showing the experimental data and the calculated straight line.
(c) An unknown sample gave an observed fluorescence intensity of 15.4.
Calculate the concentration of Riboflavin (Vitamin B2) in the unknown sample (μg/mL).
(d) Calculate the coefficient of determination (R2).
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