Rhodamine B is useful dye prepared in manner very similar to fluorescein. Due to the nitrogen donating groups, rhodamine B absorbs 550 nm light and fluoresces 580 nm light. a. What color would you expect for a rhodamine B solution? b. What is the color of the fluoresced (emitted light)? c. Provide a detailed mechanism for the formation of Rhodamine B from m- (diethylamino)phenol and phthalic anhydride in the presence of acid and heat. 2 наук of of + m-(diethylamino)phenol phthalic anhydride H₂SO4 (cat.) Heat Rhodamine B

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4. Rhodamine B is useful dye prepared in a manner very similar to fluorescein. Due to the
nitrogen donating groups, rhodamine B absorbs 550 nm light and fluoresces 580 nm light.
a. What color would you expect for a rhodamine B solution?
b. What is the color of the fluoresced (emitted light)?
c. Provide a detailed mechanism for the formation of Rhodamine B from m-
(diethylamino)phenol and phthalic anhydride in the presence of acid and heat.
2
НО.
سوم
m-(diethylamino)phenol phthalic anhydride
H₂SO4 (cat.)
Heat
Rhodamine B
Transcribed Image Text:4. Rhodamine B is useful dye prepared in a manner very similar to fluorescein. Due to the nitrogen donating groups, rhodamine B absorbs 550 nm light and fluoresces 580 nm light. a. What color would you expect for a rhodamine B solution? b. What is the color of the fluoresced (emitted light)? c. Provide a detailed mechanism for the formation of Rhodamine B from m- (diethylamino)phenol and phthalic anhydride in the presence of acid and heat. 2 НО. سوم m-(diethylamino)phenol phthalic anhydride H₂SO4 (cat.) Heat Rhodamine B
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