QUESTIONS 1) How will the following affect the TLC separation? a) too much sample applied. b) Stationary phase is too active. c) Forgetting to remove the plate when the solvent has reached the top of the plate. d) Having too much solvent in the developing chamber so that its surface extends above the origin. e) Polarity of the solvent being too high. 2) Which dye would be more soluble in n-propyl alcohol, the blue or the yellow? How can you tell? 3) Which compound is more strongly adsorbed on silica, ortho- or para- nitroaniline? Correlate the R, values with the structures.

QUESTIONS 1) How will the following affect the TLC separation? a) too much sample applied. b) Stationary phase is too active. c) Forgetting to remove the plate when the solvent has reached the top of the plate. d) Having too much solvent in the developing chamber so that its surface extends above the origin. e) Polarity of the solvent being too high. 2) Which dye would be more soluble in n-propyl alcohol, the blue or the yellow? How can you tell? 3) Which compound is more strongly adsorbed on silica, ortho- or para- nitroaniline? Correlate the R, values with the structures.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

if you wanted to makes the spots travel up the TLC plate further, what solvent ration would you choose and why? 1:2 Ethyl acetate/Hexane, 2:3 Ethyl acetate/ Hexane, or 1:1 Ethyl acetate/Hexane
Task: You are a forensic analyst employed at the Baltimore County Forensic Lab. You plan to perform paper chromatography on the ink from the note and a sample from the marker. Permanent marker ink is non-polar. You need to select the best solvent to use as the mobile phase in order to compare the ink sample and determine if they may be a match. The solvents you have available to you are carbon tetrachloride and water.Which solvent would be best to analyze the ink samples using paper chromotography?Your explanation should include:• the name of the liquid solvent that should be used for the paper chromatography analysis• scientific reasoning for your choice of the liquid solvent that will be used• the Lewis structures for carbon tetrachloride, CCl4, and water, H2O• how the shape and symmetry of each molecule influences its polarity
What are the retention times and areas of all the attached peaks?
A mixture of tert-butylbenzene and acetophenone is adsorbed onto a silica gel chromatography column and eluted with a moderately polar solvent such as dichloromethane. Match each statement to the appropriate category. least polar tert-Butylbenzene elutes first most polar elutes last Answer Bank Acetophenone
Explain why the dye spots are elongated and not round on your chromatogram.
Calculate the Rf value for a spot in a TLC experiment if the solvent moved 13.5 cm and the spot moved 9.1 cm from the origin. Type answer:
Put all steps (below) of a TLC analysis in a correct order. Write the two main visualization methods as titles a. Developing the Plate b. Calculating Rf Values c. Spotting the TLC Plate Visualizing the Results e. Preparing the Sample f. Picking a Solvent
Question 5 Which part of the HPLC contains majority of the solvent? fraction collector O reservoir O pre-column O analytical column
Consider the TLC plate pictured below. What is the Rf of the compound if the compound distance is 2.77 cm and the solvent front distance is 5.85 cm? Please report your answer to 2 decimal places only!
Draw a magnified diagram of a TLC plate in a developing chamber and indicate the following a) developing solvent b) TLC plate c) original spot d) solvent front and e) compounds beginning to separate on the TLC plate.
5. Summarize your conclusions from the TLC simulator. Circle the correct answer in each description about TLC separation of organic compounds on silica gel plates. a) A more polar compound has a lower/higher retention factor. b) A less polar compound has a lower/higher retention factor. c) A more polar solvent causes a lower/higher retention factor. d) A less polar solvent causes a lower/higher retention factor. e) To increase the retention factor of a spot, you should add more ethyl acetate/hexane.
Organic Chemistry, Column questions