Question 7: A. Calculate how to make 10 ml of a 7.5% methylene blue stock solution (from solid powder). B. Calculate how to make a series of 1:10 dilutions of this stock. Imagine you'll use 5 ml of each dilution for your experiment. Calculate the final concentrations of each tube. tube 1: take some stock, dilute it 1:10 with some water in a new tube - #1 tube 2: take some of #1, dilute it 1:10 with some water in a new tube tube 3: take some of #2, dilute it 1:10 with some water in a new tube tube 4: take some of #3, dilute it 1:10 with some water in a new tube C. First, decide the total volume to make of each tube-keep in mind measurement accuracy (consider preparing 20% more solution than what you will use). D. Then, calculate how much stock, water and final concentration of each. E. Then, instead of doing a serial dilution, calculate making each of the above solutions using the weight/volume % solution method. F. Compare and contrast serial dilution and W/V % methods

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Stress

Stress is defined as the feeling of being overwhelmed or unable to cope with emotional or mental pressure. Any event or action that makes a person annoyed, furious, or nervous can trigger it. Chemical changes in the body caused by stress can cause blood pressure, blood sugar levels, and heart rate to rise.