Question 15 Caffeine, benzoate, and aspartame content of mountain dew soda was determined using reverse phase HPLC. The concentration of the standard used are as follows, caffeine: 0.70 mg/mL, benzoate: 1.6 mg/mL, and aspartame 5.0 mg/mL. The following volume of the standards were taken to a 50.0 mL volumetric flask and diluted to the mark to construct a calibration curve: Caffeine Benzoate Aspartame 1 mL 1 mL 1 mL 2 mL 2 mL 2 mL 3 mL 3 mL 3 mL 4 mL 4 mL 4 mL 5 mL 5 mL 5 mL The 12-oz can mountain dew sample were treated as follows: The 12- oz can were has been left out overnight to get rid the carbonation then, 2 mL were drawn into a plastic syringe, filtered, and placed into a vial. An equal amount of deionized water was added. A 100 μL sample were injected into a sample loop using same parameters with the standards. Assume 12 oz - 354.9 mL The following data were obtained: Peak Area Benzoate Caffeine Aspartame Std 1 2.30 3.54 3.33 Std 2 4.10 5.59 5.83 Std 3 8. CD 7.37 9.98 9.63 Std 4 9.56 14.60 12.78 Std 5 12.18 18.16 16.33 Mountain Dew 10.21 14.97 7.96 Calculate the concentration of caffeine (mg/can) in the sample. OA 142.186 mg/can OB. 21.046 mg/can OC. 50.113 mg/can OD. 42.091 mg/can

Question 15 Caffeine, benzoate, and aspartame content of mountain dew soda was determined using reverse phase HPLC. The concentration of the standard used are as follows, caffeine: 0.70 mg/mL, benzoate: 1.6 mg/mL, and aspartame 5.0 mg/mL. The following volume of the standards were taken to a 50.0 mL volumetric flask and diluted to the mark to construct a calibration curve: Caffeine Benzoate Aspartame 1 mL 1 mL 1 mL 2 mL 2 mL 2 mL 3 mL 3 mL 3 mL 4 mL 4 mL 4 mL 5 mL 5 mL 5 mL The 12-oz can mountain dew sample were treated as follows: The 12- oz can were has been left out overnight to get rid the carbonation then, 2 mL were drawn into a plastic syringe, filtered, and placed into a vial. An equal amount of deionized water was added. A 100 μL sample were injected into a sample loop using same parameters with the standards. Assume 12 oz - 354.9 mL The following data were obtained: Peak Area Benzoate Caffeine Aspartame Std 1 2.30 3.54 3.33 Std 2 4.10 5.59 5.83 Std 3 8. CD 7.37 9.98 9.63 Std 4 9.56 14.60 12.78 Std 5 12.18 18.16 16.33 Mountain Dew 10.21 14.97 7.96 Calculate the concentration of caffeine (mg/can) in the sample. OA 142.186 mg/can OB. 21.046 mg/can OC. 50.113 mg/can OD. 42.091 mg/can

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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