Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
Question:
1. Enumerate the different application of Dyna Bead Analysis.
2. Discuss the significance of this method.
Transcribed Image Text:Dynabead Applications for Genome Analysis
Applications include: Differential display, 5'RACE, S1 nuclease mapping, DNase footprinting,
purification of RNA/DNA binding proteins, and subtractive hybridization for isolation of tumor
specific alterations and identification of toxin genes in pathogens.
Streptavidin-Coupled Dynabeads or Dynabeads Oligo (dT)25 (Cat. Nos. 61002, 61005) are used
in many gene expression profiling methods, e.g. based on representational cloning and
sequencing of cDNAs from libraries.
Double stranded DNA sequences longer than 2 kb are effectively immobilized with
the Dynabeads kilobaseBINDER Kit.
Streptavidin-coupled Dynabeads magnetic beads used in a RTPCR/ECL assay provide a
practical and sensitive approach for detection of various metastatic cancers in tissues and
blood.
The beads have also been cited in numerous published articles on the SAGE® technology and
in a related method published by S. Brenner et
IV.
QUESTIONS:
1. Enumerate the different application of Dyna Bead Analysis.
2. Discuss the significance of this method.
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