Q3 We want to measure the concentration of a molecule in water that absorbs green light. When a sample solution is illuminated with a green laser from one side of the cuvette we measure green intensity that leaves the cuvette on the opposite side with a detector. 1. What is intensity? 2. The ratio of the intensity of illumination entering the cuvette relative to the intensity that is detected by the detector we obtain 1.2 which can be expressed with transmittance. What is the value of transmittance here? 3. What is the value of absorbance?

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sample solution is illuminated with a green laser from one side of the cuvette we measure green intensity
that leaves the cuvette on the opposite side with a detector.
We want to measure the concentration of a molecule in water that absorbs green light. When a
1. What is intensity?
2. The ratio of the intensity of illumination entering the cuvette relative to the intensity that is
detected by the detector we obtain 1.2 which can be expressed with transmittance. What is the
value of transmittance here?
3. What is the value of absorbance?
4. We dilute the sample with water such that the concentration of the green molecule decreases to
1/10th of the original concentration. What is the absorbance measured in this sample? What is the
transmittance?
Transcribed Image Text:Q3 sample solution is illuminated with a green laser from one side of the cuvette we measure green intensity that leaves the cuvette on the opposite side with a detector. We want to measure the concentration of a molecule in water that absorbs green light. When a 1. What is intensity? 2. The ratio of the intensity of illumination entering the cuvette relative to the intensity that is detected by the detector we obtain 1.2 which can be expressed with transmittance. What is the value of transmittance here? 3. What is the value of absorbance? 4. We dilute the sample with water such that the concentration of the green molecule decreases to 1/10th of the original concentration. What is the absorbance measured in this sample? What is the transmittance?
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