Q1.Fill in positive, negative and neutral The isoelectric point, pl, of two amino acids is shown below Amino acid A Amino acid B H,N-CH-C-OH CH2 он ksoelectric point = 63 isoelectric point = 9.5 What is the likely net charge of amino acid A at pH 7.8 What is the likely net charge of amino acid B at pH 6.5 Q2. The side groups of proteins can react with carbohydrates to form glycoproteins. The reaction occurs through a nucleophilic group on the side group of an amino acid residue. As part of a protein, which of the following amino acid residues is NOT appropriate to react with a carbohydrate molecule.

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Chapter1: Chemical Foundations
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Q1.Fill in positive, negative and neutral
The isoelectric point, pl, of two amino acids is shown below
Amino acid A
Amino acid B
H,N-CH-C -OH
он
CH,
OH
isoelectric point = 6.3
isoelectric point = 9.5
What is the likely net charge of amino acid A at pH 7.8
What is the likely net charge of amino acid B at pH 6.5
Q2.
The side groups of proteins can react with carbohydrates to
form glycoproteins. The reaction occurs through a nucleophilic
group on the side group of an amino acid residue.
As part of a protein, which of the following amino acid residues
is NOT appropriate to react with a carbohydrate molecule.
H O
HạN-C-C-OH
Lysine
NH2
Ob.
H O
HN-C
-C
HO-
CH3
Alanine
Oc.
H
H,N-C-C-OH
но.
Serine
Od.
H,N-C-C-OH
он
Threonine
Q3.
The isoelectric point of a molecule can be calculated from the
pk, values of its ionisable groups.
Calculate the isoelectric point of the molecule below.
pk, = 3.7
HO
OH
pk, = 2.1
pk, = 9.8
Give your answer to one decimal point.
Answer.
Transcribed Image Text:Q1.Fill in positive, negative and neutral The isoelectric point, pl, of two amino acids is shown below Amino acid A Amino acid B H,N-CH-C -OH он CH, OH isoelectric point = 6.3 isoelectric point = 9.5 What is the likely net charge of amino acid A at pH 7.8 What is the likely net charge of amino acid B at pH 6.5 Q2. The side groups of proteins can react with carbohydrates to form glycoproteins. The reaction occurs through a nucleophilic group on the side group of an amino acid residue. As part of a protein, which of the following amino acid residues is NOT appropriate to react with a carbohydrate molecule. H O HạN-C-C-OH Lysine NH2 Ob. H O HN-C -C HO- CH3 Alanine Oc. H H,N-C-C-OH но. Serine Od. H,N-C-C-OH он Threonine Q3. The isoelectric point of a molecule can be calculated from the pk, values of its ionisable groups. Calculate the isoelectric point of the molecule below. pk, = 3.7 HO OH pk, = 2.1 pk, = 9.8 Give your answer to one decimal point. Answer.
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