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Proteins are characterized by their isoelectric point (pl), at which they carry no net charge. Proteins dissolved in a buffer solution with pH larger than the isoelectric point are negatively charged and positively charged when they are present in solution with a pH smaller than their isoelectric point. Consider a protein mixture consisting of protein A with pl-5.1 (MW 10,000), protein B with pl-7.1 (MW 10,000) protein C with pl-9.6 (MW 10,000) which will be separated by Capillary Zone Electrophoresis. Determine the migration order (fastest to slowest) in a buffer solution at neutral pH (negative potential applied to outlet and positive potential applied to the inlet, assume negative charge at capillary wall).

Proteins are characterized by their isoelectric point (pl), at which they carry no net charge. Proteins dissolved in a buffer solution with pH larger than the isoelectric point are negatively charged and positively charged when they are present in solution with a pH smaller than their isoelectric point. Consider a protein mixture consisting of protein A with pl-5.1 (MW 10,000), protein B with pl-7.1 (MW 10,000) protein C with pl-9.6 (MW 10,000) which will be separated by Capillary Zone Electrophoresis. Determine the migration order (fastest to slowest) in a buffer solution at neutral pH (negative potential applied to outlet and positive potential applied to the inlet, assume negative charge at capillary wall).

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Proteins are characterized by their isoelectric point (pl), at which they carry no net charge. Proteins dissolved in a buffer solution with pH larger than the isoelectric point are negatively charged and positively charged when they are present in solution with a pH smaller than their isoelectric point. Consider a protein mixture consisting of protein A with pl=5.1 (MW 10,000), protein B with pl=7.1 (MW 10,000) protein C with pl=9.6 (MW 10,000) which will be separated by Capillary Zone Electrophoresis. Determine the migration order (fastest to slowest) in a buffer solution at neutral pH (negative potential applied to outlet and positive potential applied to the inlet, assume negative charge at capillary wall). O A>B>C O A=B=C O AcC<8 O C-B>A
Transcribed Image Text:Proteins are characterized by their isoelectric point (pl), at which they carry no net charge. Proteins dissolved in a buffer solution with pH larger than the isoelectric point are negatively charged and positively charged when they are present in solution with a pH smaller than their isoelectric point. Consider a protein mixture consisting of protein A with pl=5.1 (MW 10,000), protein B with pl=7.1 (MW 10,000) protein C with pl=9.6 (MW 10,000) which will be separated by Capillary Zone Electrophoresis. Determine the migration order (fastest to slowest) in a buffer solution at neutral pH (negative potential applied to outlet and positive potential applied to the inlet, assume negative charge at capillary wall). O A>B>C O A=B=C O AcC<8 O C-B>A
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