Preparing solutions with a defined pH, ionic strength and solute concentration(s) is a vital laboratory skill as the stability and conformation of isolated organelles and biomolecules are often sensitive to each of these variables. You will prepare 100. ml of a solution that contains each of the following: 22.5 µM ATP and a total concentration of 50.0 mM Tris that is buffered to a final pH of 7.70. NOTE: Tris is the abbreviation for the weak base tris (hydroxymethyl) aminomethane. Before lab starts, calculate the masses of Tris base and Tris Cl needed to make this solution. Also calculate the volume of a 25.0 mM ATP stock solution that is needed to make this solution. Some potentially useful information: Solid Tris CI, the conjugate acid of Tris with a pk, of 8.10 (at 25°C) and FW = 157.6 Solid Tris base, FW = 121.1 %3D Materials Needed g Tris Cl g Tris base mL 25.0 mM ATP stock solution 5 mL of Reference solution 50 mM Tris buffer, pH 7.70) 10 mL of pH 7.00 buffer to calibrate your pH meter
Preparing solutions with a defined pH, ionic strength and solute concentration(s) is a vital laboratory skill as the stability and conformation of isolated organelles and biomolecules are often sensitive to each of these variables. You will prepare 100. ml of a solution that contains each of the following: 22.5 µM ATP and a total concentration of 50.0 mM Tris that is buffered to a final pH of 7.70. NOTE: Tris is the abbreviation for the weak base tris (hydroxymethyl) aminomethane. Before lab starts, calculate the masses of Tris base and Tris Cl needed to make this solution. Also calculate the volume of a 25.0 mM ATP stock solution that is needed to make this solution. Some potentially useful information: Solid Tris CI, the conjugate acid of Tris with a pk, of 8.10 (at 25°C) and FW = 157.6 Solid Tris base, FW = 121.1 %3D Materials Needed g Tris Cl g Tris base mL 25.0 mM ATP stock solution 5 mL of Reference solution 50 mM Tris buffer, pH 7.70) 10 mL of pH 7.00 buffer to calibrate your pH meter
Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:Introduction
Preparing solutions with a defined pH, ionic strength and solute concentration(s) is a vital
laboratory skill as the stability and conformation of isolated organelles and biomolecules are
often sensitive to each of these variables. You will prepare 100. mL of a solution that contains
each of the following: 22.5 µM ATP and a total concentration of 50.0 mM Tris that is buffered to
a final pH of 7.70.
NOTE: Tris is the abbreviation for the weak base tris (hydroxymethyl) aminomethane.
Before lab starts, calculate the masses of Tris base and Tris Cl needed to make this solution.
Also calculate the volume of a 25.0 mM ATP stock solution that is needed to make this solution.
Some potentially useful information:
Solid Tris CI, the conjugate acid of Tris with a pK, of 8.10 (at 25°C) and FW = 157.6
Solid Tris base, FW = 121.1
%3D
Materials Needed
g Tris CI
g Tris base
mL 25.0 mM ATP stock solution
5 mL of Reference solution 50 mM Tris buffer, pH 7.70)
10 ml of pH 7.00 buffer to calibrate your pH meter
Expert Solution
Analysis
Given : volume of solution =100 ml. Final PH of solution = 7.70
To find: mass of tris Cl and mass of tris Base.
Solution : As we know that, molarity of a solution depends upon the moles of solute and volume of solution and also moles of solute depend upon the mass of solute and molar mass of solute.
Mole =mass / molar mass
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