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phics Integration Calibration Report Batch MOCHEM 2 Caff M (sequence) 025-02-04 08-52-23 8-52-23_002-53-AM.PDF View Abort Help MAU VWD1 A, Wavelength-272 nm (002-53-AM.D) 400 300 200 100- 0 ============ 0.971 112 1.318 1.552 Area Percent Report Sorted By Multiplier Dilution : Signal 1.0000 : 1.0000 CLC (online): Data A... AMI Line: 21 Inj... 2.144 2 Max open SYSTEM localhost Post Lab Questions 1. (0.5pts) How many compounds were there in the given Caffeine sample? 2. (0.5 pts) What is the retention time of Caffeine in the chromatogram obtained? 3. (1 pts) What is the percentage purity of Caffeine sample you prepared? 4. (1 pts) what are the possible sources of errors that can lead to lower percentage purity of the sample. 5. (1 pts) What is the mobile phase used in your experiment? 6. (1 pts) What is the basic principle involved in reverse phase HPLC? 7. (1 pts) Mention any 2 applications of HPLC. 8. (1 pts) What happens to the retention time of Caffeine if you change the mobile phase to 100% Methanol (Retention time would increase or decrease)? Why? 9. (1 pts) What happens to the retention time of Caffeine if you change the mobile phase to 100% water (Retention time would increase or decrease)? Why? 10. (1 pts) What happens to the retention time of Caffeine if you change the runtime to 28 minutes (Retention time would increase or decrease)? Why? 11. (1 pts) What happens to the signal shape if the concentration of the injected Caffeine sample is 2 M instead of 0.02 M. Data

phics Integration Calibration Report Batch MOCHEM 2 Caff M (sequence) 025-02-04 08-52-23 8-52-23_002-53-AM.PDF View Abort Help MAU VWD1 A, Wavelength-272 nm (002-53-AM.D) 400 300 200 100- 0 ============ 0.971 112 1.318 1.552 Area Percent Report Sorted By Multiplier Dilution : Signal 1.0000 : 1.0000 CLC (online): Data A... AMI Line: 21 Inj... 2.144 2 Max open SYSTEM localhost Post Lab Questions 1. (0.5pts) How many compounds were there in the given Caffeine sample? 2. (0.5 pts) What is the retention time of Caffeine in the chromatogram obtained? 3. (1 pts) What is the percentage purity of Caffeine sample you prepared? 4. (1 pts) what are the possible sources of errors that can lead to lower percentage purity of the sample. 5. (1 pts) What is the mobile phase used in your experiment? 6. (1 pts) What is the basic principle involved in reverse phase HPLC? 7. (1 pts) Mention any 2 applications of HPLC. 8. (1 pts) What happens to the retention time of Caffeine if you change the mobile phase to 100% Methanol (Retention time would increase or decrease)? Why? 9. (1 pts) What happens to the retention time of Caffeine if you change the mobile phase to 100% water (Retention time would increase or decrease)? Why? 10. (1 pts) What happens to the retention time of Caffeine if you change the runtime to 28 minutes (Retention time would increase or decrease)? Why? 11. (1 pts) What happens to the signal shape if the concentration of the injected Caffeine sample is 2 M instead of 0.02 M. Data

EBK A SMALL SCALE APPROACH TO ORGANIC L
EBK A SMALL SCALE APPROACH TO ORGANIC L
4th Edition
ISBN:9781305446021
Author:Lampman
Publisher:Lampman
Chapter88: Column Chromatography
Section: Chapter Questions
Problem 5P
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phics Integration Calibration Report Batch MOCHEM 2 Caff M (sequence) 025-02-04 08-52-23 8-52-23_002-53-AM.PDF View Abort Help MAU VWD1 A, Wavelength-272 nm (002-53-AM.D) 400 300 200 100- 0 ============ 0.971 112 1.318 1.552 Area Percent Report Sorted By Multiplier Dilution : Signal 1.0000 : 1.0000 CLC (online): Data A... AMI Line: 21 Inj... 2.144 2 Max open SYSTEM localhost
Transcribed Image Text:phics Integration Calibration Report Batch MOCHEM 2 Caff M (sequence) 025-02-04 08-52-23 8-52-23_002-53-AM.PDF View Abort Help MAU VWD1 A, Wavelength-272 nm (002-53-AM.D) 400 300 200 100- 0 ============ 0.971 112 1.318 1.552 Area Percent Report Sorted By Multiplier Dilution : Signal 1.0000 : 1.0000 CLC (online): Data A... AMI Line: 21 Inj... 2.144 2 Max open SYSTEM localhost
Post Lab Questions 1. (0.5pts) How many compounds were there in the given Caffeine sample? 2. (0.5 pts) What is the retention time of Caffeine in the chromatogram obtained? 3. (1 pts) What is the percentage purity of Caffeine sample you prepared? 4. (1 pts) what are the possible sources of errors that can lead to lower percentage purity of the sample. 5. (1 pts) What is the mobile phase used in your experiment? 6. (1 pts) What is the basic principle involved in reverse phase HPLC? 7. (1 pts) Mention any 2 applications of HPLC. 8. (1 pts) What happens to the retention time of Caffeine if you change the mobile phase to 100% Methanol (Retention time would increase or decrease)? Why? 9. (1 pts) What happens to the retention time of Caffeine if you change the mobile phase to 100% water (Retention time would increase or decrease)? Why? 10. (1 pts) What happens to the retention time of Caffeine if you change the runtime to 28 minutes (Retention time would increase or decrease)? Why? 11. (1 pts) What happens to the signal shape if the concentration of the injected Caffeine sample is 2 M instead of 0.02 M. Data
Transcribed Image Text:Post Lab Questions 1. (0.5pts) How many compounds were there in the given Caffeine sample? 2. (0.5 pts) What is the retention time of Caffeine in the chromatogram obtained? 3. (1 pts) What is the percentage purity of Caffeine sample you prepared? 4. (1 pts) what are the possible sources of errors that can lead to lower percentage purity of the sample. 5. (1 pts) What is the mobile phase used in your experiment? 6. (1 pts) What is the basic principle involved in reverse phase HPLC? 7. (1 pts) Mention any 2 applications of HPLC. 8. (1 pts) What happens to the retention time of Caffeine if you change the mobile phase to 100% Methanol (Retention time would increase or decrease)? Why? 9. (1 pts) What happens to the retention time of Caffeine if you change the mobile phase to 100% water (Retention time would increase or decrease)? Why? 10. (1 pts) What happens to the retention time of Caffeine if you change the runtime to 28 minutes (Retention time would increase or decrease)? Why? 11. (1 pts) What happens to the signal shape if the concentration of the injected Caffeine sample is 2 M instead of 0.02 M. Data
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