Plot absorption versus time using the graph label mAbs vs time(s) from 0 to 120 s in 10-s intervals. Plot a line that can be used to calculate the initial rate using the graph label Initial Rate.
Plot absorption versus time using the graph label mAbs vs time(s) from 0 to 120 s in 10-s intervals. Plot a line that can be used to calculate the initial rate using the graph label Initial Rate.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Plot absorption versus time using the graph label mAbs vs time(s) from 0 to 120 s in 10-s intervals. Plot a line that can be used to calculate the initial rate using the graph label Initial Rate.
Transcribed Image Text:In your first kinetic experiment, you decide to assess the enzyme's ability to hydrolyze the 8-lactam amoxicillin.
The hydrolysis of amoxicillin can be observed directly by following the change in absorbance at 240 nm. Kinetic assays are performed at 30 °C in a buffer of 100 µM sodium phosphate (pH 7.0). Starting with 50
µM amoxicillin, you measure the absorbance at 240 nm every 10 s for two minutes and obtain the following values:
Time
Absorbance
Time
Absorbance
(s)
(mAbs)
(s)
(mAbs)
542.5
70
323,9
10
508.5
80
300.5
20
474.4
278.8
90
30
440.4
100
258.6
40
406.4
110
239.9
50
376.3
120
222.6
60
349.1
Given the data for amoxicillin, plot absorbance versus time (mAbs vs time (s)) for an initial concentration of 50 µM. Then, add a line that can be used to calculate the initial rate of the reaction (Initial Rate).
Transcribed Image Text:No elements selected
Absorbance
(mAbe)
550
525
S0
475
450
425
O--- ----- ..
400
375
350
325
300
275
250
225
10
30
40
50
60
70
80
90
100
110
120
time(s)
20
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