please help me make this procedure a flow chart III. Procedure: A. Microscopy Operating Procedure: Place the microscope close to the edge of the table. Select a suitable stool so that when looking into the eyepiece, your back is straight and your neck is bent at the nape. 1. Lower the body tube by turning the course focus knob until the 10x or 16mm objective reaches the downward stop. 2. Look through the eyepiece and adjust the mirror to the position which provides the brightest and the most evenly illuminated field of vision which is the circular area seen in the eyepiece. Raise the condenser until its top lens at the same level as the stage. Place the slide on the stage and fasten it using the stage clips. 3. Position the specimen area of the slide over the center of the stage aperture. 4. Looking through the eyepiece, raise the coarse focus knob until the image appears. Focus as sharply as possible. Low power objective has a much greater depth of focus and is generally used for the initial focusing and viewing. 5. Adjust the fine focus knob to sharpen the image in the center of the field of vision. 6. When a feature on the specimen is to be examined at a higher magnification, move the slide so that the feature is centered in the field of vision. The higher the power of the objective, the lesser is the area of the specimen surface included in the field of vision. Shift the higher powered objective into place and adjust with the fine focus knob. 7. The eye should be kept at a certain distance from the eyepiece. Be relaxed when looking into the eyepiece and keep both eyes open. Note: on your worksheet, label the different parts of a compound microscope.
please help me make this procedure a flow chart
III. Procedure:
A. Microscopy
Operating Procedure:
Place the microscope close to the edge of the table. Select a suitable stool so
that when looking into the eyepiece, your back is straight and your neck is
bent at the nape.
1. Lower the body tube by turning the course focus knob until the 10x or
16mm objective reaches the downward stop.
2. Look through the eyepiece and adjust the mirror to the position which
provides the brightest and the most evenly illuminated field of vision which
is the circular area seen in the eyepiece. Raise the condenser until its top
lens at the same level as the stage. Place the slide on the stage and
fasten it using the stage clips.
3. Position the specimen area of the slide over the center of the stage
aperture.
4. Looking through the eyepiece, raise the coarse focus knob until the image
appears. Focus as sharply as possible. Low power objective has a much
greater depth of focus and is generally used for the initial focusing and
viewing.
5. Adjust the fine focus knob to sharpen the image in the center of the field of
vision.
6. When a feature on the specimen is to be examined at a higher
magnification, move the slide so that the feature is centered in the field of
vision. The higher the power of the objective, the lesser is the area of the
specimen surface included in the field of vision. Shift the higher powered
objective into place and adjust with the fine focus knob.
7. The eye should be kept at a certain distance from the eyepiece. Be
relaxed when looking into the eyepiece and keep both eyes open.
Note: on your worksheet, label the different parts of a compound microscope.
Take note also of the characteristics of your microscope and fill up table 1.
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