Phosphate buffered saline (PBS) is a physiological buffer often used in biology experiments. Because the concentrations are very low and small amounts are difficult to weigh out, it is often prepared as a 10X stock solution meaning that it is prepared at 10 times the working concentration which is 1X. 1X PBS is made up of Na2HPO4 10 mM (MW 141.9 g.mol-1); KH2PO4 8 mM (MW 136.08 g.mol-1), NaCl 137 mM (MW 58.44 g.mol-1) and KCl 74.5 mM (MW 74.5 g.mol-1). You decide to prepare a 1 L 10X solution of PBS. What is the Molar (M) concentration of each of the components in you 10X stock. Write your answers in the table below.How much of each of the components should you weigh out to prepare 1L of the 10X stock solutions. Write your answers in the table below. ChemicalConcentrationConcentrationAmount requiredComponent|(1X)|(10X)|(g)Na2HPO410 mMKH2PO41.8 mMNaCI137 mMKCI|74.5 mM

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Human Anatomy & Physiology (11th Edition)

11th Edition

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Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

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Phosphate buffered saline (PBS) is a physiological buffer often used in biology experiments. Because the concentrations are very low and small amounts are difficult to weigh out, it is often prepared as a 10X stock solution meaning that it is prepared at 10 times the working concentration which is 1X. 1X PBS is made up of Na₂HPO₄ 10 mM (MW 141.9 g.mol^-1); KH₂PO₄8 mM (MW 136.08 g.mol^-1), NaCl 137 mM (MW 58.44 g.mol^-1) and KCl 74.5 mM (MW 74.5 g.mol^-1).

You decide to prepare a 1 L 10X solution of PBS. What is the Molar (M) concentration of each of the components in you 10X stock. Write your answers in the table below.
How much of each of the components should you weigh out to prepare 1L of the 10X stock solutions. Write your answers in the table below.

Chemical
Concentration
Concentration
Amount required
Component
|(1X)
|(10X)
|(g)
Na2HPO4
10 mM
KH2PO4
1.8 mM
NaCI
137 mM
KCI
|74.5 mM

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