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Part 1. Experiment to determine the specific binding of [3H]-flunitrazepam as defined by three different competing ligands Total binding Non-specific binding Specific binding c.p.m. 120.67 Total binding c.p.m. 143.00 Total binding c.p.m. 138 Diazepam c.p.m. 110.67 Flunitrazepam 128.00 2416.00 Non-specific binding Specific binding c.p.m. c.p.m. 133.00 c.p.m. c.p.m. Midazolam 2393.67 Non-specific binding Specific binding c.p.m. 2398.67 % Specific binding 95.24310118 % Specific binding 94.36268068 % Specific binding 94.55978975 The receptor or drug target in this case was the rat GABAA receptor obtained from homogenised rat brain cortical tissue. Using the same radioligand binding technique, what other way could you look at ligand affinity to GABAA receptors without using GABAA receptors derived from homogenised tissue? What are the advantages/disadvantages to each strategy?

Part 1. Experiment to determine the specific binding of [3H]-flunitrazepam as defined by three different competing ligands Total binding Non-specific binding Specific binding c.p.m. 120.67 Total binding c.p.m. 143.00 Total binding c.p.m. 138 Diazepam c.p.m. 110.67 Flunitrazepam 128.00 2416.00 Non-specific binding Specific binding c.p.m. c.p.m. 133.00 c.p.m. c.p.m. Midazolam 2393.67 Non-specific binding Specific binding c.p.m. 2398.67 % Specific binding 95.24310118 % Specific binding 94.36268068 % Specific binding 94.55978975 The receptor or drug target in this case was the rat GABAA receptor obtained from homogenised rat brain cortical tissue. Using the same radioligand binding technique, what other way could you look at ligand affinity to GABAA receptors without using GABAA receptors derived from homogenised tissue? What are the advantages/disadvantages to each strategy?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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x₂ x² A2 A V Part 1. Experiment to determine the specific binding of [3H]-flunitrazepam as defined by three different competing ligands I Total binding c.p.m. Total binding Non-specific binding Specific binding % Specific binding c.p.m. 120.67 143.00 Total binding c.p.m. 138 c.p.m. English (United Kingdom) 110.67 Diazepam Flunitrazepam c.p.m. 133.00 128.00 Non-specific binding Specific binding c.p.m. Non-specific binding c.p.m. 2416.00 c.p.m. Midazolam ♫ 2393.67 ♥ Specific binding c.p.m. 2398.67 95.24310118 átv A % Specific binding 94.36268068 % Specific binding The receptor or drug target in this case was the rat GABAA receptor obtained from homogenised rat brain cortical tissue. Using the same radioligand binding technique, what other way could you look at ligand affinity to GABAA receptors without using GABAA receptors derived from homogenised tissue? What are the advantages/disadvantages to each strategy? 94.55978975 Styles St P Focus each strategy? N E X
Transcribed Image Text:x₂ x² A2 A V Part 1. Experiment to determine the specific binding of [3H]-flunitrazepam as defined by three different competing ligands I Total binding c.p.m. Total binding Non-specific binding Specific binding % Specific binding c.p.m. 120.67 143.00 Total binding c.p.m. 138 c.p.m. English (United Kingdom) 110.67 Diazepam Flunitrazepam c.p.m. 133.00 128.00 Non-specific binding Specific binding c.p.m. Non-specific binding c.p.m. 2416.00 c.p.m. Midazolam ♫ 2393.67 ♥ Specific binding c.p.m. 2398.67 95.24310118 átv A % Specific binding 94.36268068 % Specific binding The receptor or drug target in this case was the rat GABAA receptor obtained from homogenised rat brain cortical tissue. Using the same radioligand binding technique, what other way could you look at ligand affinity to GABAA receptors without using GABAA receptors derived from homogenised tissue? What are the advantages/disadvantages to each strategy? 94.55978975 Styles St P Focus each strategy? N E X
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