Part 1. Experiment to determine the specific binding of [3H]-flunitrazepam as defined by three different competing ligands Total binding Non-specific binding Specific binding c.p.m. 120.67 Total binding c.p.m. 143.00 Total binding c.p.m. 138 c.p.m. 110.67 Diazepam c.p.m. 128.00 2416.00 Flunitrazepam Non-specific binding Specific binding c.p.m. 133.00 c.p.m. Midazolam c.p.m. 2393.67 Non-specific binding Specific binding c.p.m. 2398.67 % Specific binding 95.24310118 % Specific binding 94.36268068 % Specific binding 94.55978975 Saturation experiments using radioligand is one method of determining the affinity of a ligand to a drug target. What are other methods used in high-throughput screening (HTS) of compound libraries that do not use a radioligand?
Part 1. Experiment to determine the specific binding of [3H]-flunitrazepam as defined by three different competing ligands Total binding Non-specific binding Specific binding c.p.m. 120.67 Total binding c.p.m. 143.00 Total binding c.p.m. 138 c.p.m. 110.67 Diazepam c.p.m. 128.00 2416.00 Flunitrazepam Non-specific binding Specific binding c.p.m. 133.00 c.p.m. Midazolam c.p.m. 2393.67 Non-specific binding Specific binding c.p.m. 2398.67 % Specific binding 95.24310118 % Specific binding 94.36268068 % Specific binding 94.55978975 Saturation experiments using radioligand is one method of determining the affinity of a ligand to a drug target. What are other methods used in high-throughput screening (HTS) of compound libraries that do not use a radioligand?
Chapter6: Oral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 6.8P
Related questions
Question
![A A Aa A
₂²AA.
Total binding
c.p.m.
143.00
Part 1. Experiment to determine the specific binding of [3H]-flunitrazepam
as defined by three different competing ligands
Total binding
c.p.m.
AE
138
Total binding Non-specific binding Specific binding
c.p.m.
120.67
English (United Kingdom)
====✓ | B
c.p.m.
110.67
V
Diazepam
c.p.m.
133.00
c.p.m.
128.00
Flunitrazepam
2416.00
Non-specific binding Specific binding
| ==| A | ¶
c.p.m.
Midazolam
2393.67
c.p.m.
Non-specific binding Specific binding
c.p.m.
2398.67
% Specific binding
95.24310118
% Specific binding
94.36268068
% Specific binding
94.55978975
Saturation experiments using radioligand is one method of determining the affinity of
a ligand to a drug target. What are other methods used in high-throughput screening
(HTS) of compound libraries that do not use a radioligand?
A[
Styles St
P
Focus
each strategy?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F19fb65da-94b8-41a4-a1ee-990279e142c6%2F5128bceb-c9c7-4f37-9eab-042aa6679bf5%2Fy12x6v_processed.jpeg&w=3840&q=75)
Transcribed Image Text:A A Aa A
₂²AA.
Total binding
c.p.m.
143.00
Part 1. Experiment to determine the specific binding of [3H]-flunitrazepam
as defined by three different competing ligands
Total binding
c.p.m.
AE
138
Total binding Non-specific binding Specific binding
c.p.m.
120.67
English (United Kingdom)
====✓ | B
c.p.m.
110.67
V
Diazepam
c.p.m.
133.00
c.p.m.
128.00
Flunitrazepam
2416.00
Non-specific binding Specific binding
| ==| A | ¶
c.p.m.
Midazolam
2393.67
c.p.m.
Non-specific binding Specific binding
c.p.m.
2398.67
% Specific binding
95.24310118
% Specific binding
94.36268068
% Specific binding
94.55978975
Saturation experiments using radioligand is one method of determining the affinity of
a ligand to a drug target. What are other methods used in high-throughput screening
(HTS) of compound libraries that do not use a radioligand?
A[
Styles St
P
Focus
each strategy?
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