| This problem can be worked with Equations 18-6 on a calculator or with the spreadsheet in Figure 18-5. Transferrin is the iron-transport protein found in blood. It has a molecular mass of 81 000 and carries two Fe³* ions. Desferrioxamine B is a chelator used to 18-A. treat patients with iron overload (Box 11-1). It has a molecular mass of about 650 and can bind one Fe³*. Desferrioxamine can take iron from many sites within the body and is excreted (with its iron) through the kidneys. Molar absorptivities of these compounds (satu- rated with iron) at two wavelengths are given in the table. Both com- pounds are colorless (no visible absorption) in the absence of iron. E(M' cm-') A(nm) Transferrin Desferrioxamine 428 3 540 2 730 470 4 170 2 290 (a) A solution of transferrin exhibits an absorbance of 0.463 at 470 nm in a 1.000-cm cell. Calculate the concentration of transferrin in mil- ligrams per milliliter and the concentration of bound iron in micro- grams per milliliter. (b) After adding desferrioxamine (which dilutes the sample), the absorbance at 470 nm was 0.424, and the absorbance at 428 nm was 0.401. Calculate the fraction of iron in transferrin and the fraction in desferrioxamine. Remember that transferrin binds two iron atoms and desferrioxamine binds only one.
| This problem can be worked with Equations 18-6 on a calculator or with the spreadsheet in Figure 18-5. Transferrin is the iron-transport protein found in blood. It has a molecular mass of 81 000 and carries two Fe³* ions. Desferrioxamine B is a chelator used to 18-A. treat patients with iron overload (Box 11-1). It has a molecular mass of about 650 and can bind one Fe³*. Desferrioxamine can take iron from many sites within the body and is excreted (with its iron) through the kidneys. Molar absorptivities of these compounds (satu- rated with iron) at two wavelengths are given in the table. Both com- pounds are colorless (no visible absorption) in the absence of iron. E(M' cm-') A(nm) Transferrin Desferrioxamine 428 3 540 2 730 470 4 170 2 290 (a) A solution of transferrin exhibits an absorbance of 0.463 at 470 nm in a 1.000-cm cell. Calculate the concentration of transferrin in mil- ligrams per milliliter and the concentration of bound iron in micro- grams per milliliter. (b) After adding desferrioxamine (which dilutes the sample), the absorbance at 470 nm was 0.424, and the absorbance at 428 nm was 0.401. Calculate the fraction of iron in transferrin and the fraction in desferrioxamine. Remember that transferrin binds two iron atoms and desferrioxamine binds only one.
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Transcribed Image Text:This problem can be worked with Equations 18-6 on a
calculator or with the spreadsheet in Figure 18-5. Transferrin is the
iron-transport protein found in blood. It has a molecular mass of 81 000
and carries two Fe³* ions. Desferrioxamine B is a chelator used to
18-A.
treat patients with iron overload (Box 11-1). It has a molecular mass
of about 650 and can bind one Fe³+. Desferrioxamine can take iron
from many sites within the body and is excreted (with its iron)
through the kidneys. Molar absorptivities of these compounds (satu-
rated with iron) at two wavelengths are given in the table. Both com-
pounds are colorless (no visible absorption) in the absence of iron.
e(M¯' cm¯')
A(nm)
Transferrin
Desferrioxamine
428
3 540
2 730
470
4 170
2 290
(a) A solution of transferrin exhibits an absorbance of 0.463 at 470 nm
in a 1.000-cm cell. Calculate the concentration of transferrin in mil-
ligrams per milliliter and the concentration of bound iron in micro-
grams per milliliter.
(b) After adding desferrioxamine (which dilutes the sample), the
absorbance at 470 nm was 0.424, and the absorbance at 428 nm was
0.401. Calculate the fraction of iron in transferrin and the fraction in
desferrioxamine. Remember that transferrin binds two iron atoms
and desferrioxamine binds only one.
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