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One of the challenges of studying RNA is the rapid degeneration of RNA. While the half-life of mRNA depends on the solutionit is in, in situ the half life of mRNA is between 5 minutes (E. coli) and 25 minutes (S. cerevisiae). When performing gene expression analyses it is important to make sure you can freeze a sample of mRNA in liquid nitrogen (halting mRNA decay) rapidly as to not loose too much of the original expression. A. You are doing an experiment with E. coli, what is the decay mRNA decay rate?

One of the challenges of studying RNA is the rapid degeneration of RNA. While the half-life of mRNA depends on the solutionit is in, in situ the half life of mRNA is between 5 minutes (E. coli) and 25 minutes (S. cerevisiae). When performing gene expression analyses it is important to make sure you can freeze a sample of mRNA in liquid nitrogen (halting mRNA decay) rapidly as to not loose too much of the original expression. A. You are doing an experiment with E. coli, what is the decay mRNA decay rate?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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One of the challenges of studying RNA is the rapid degeneration of RNA. While the half-life of mRNA depends on the solutionit is in, in situ the half life of mRNA is between 5 minutes (E. coli) and 25 minutes (S. cerevisiae). When performing gene expression analyses it is important to make sure you can freeze a sample of mRNA in liquid nitrogen (halting mRNA decay) rapidly as to not loose too much of the original expression. A. You are doing an experiment with E. coli, what is the decay mRNA decay rate? B. If the sample initially has 0.25 pico-moles or mRNA, how much will remain in the sam- ple if you freeze it 3 minutes later?
Transcribed Image Text:One of the challenges of studying RNA is the rapid degeneration of RNA. While the half-life of mRNA depends on the solutionit is in, in situ the half life of mRNA is between 5 minutes (E. coli) and 25 minutes (S. cerevisiae). When performing gene expression analyses it is important to make sure you can freeze a sample of mRNA in liquid nitrogen (halting mRNA decay) rapidly as to not loose too much of the original expression. A. You are doing an experiment with E. coli, what is the decay mRNA decay rate? B. If the sample initially has 0.25 pico-moles or mRNA, how much will remain in the sam- ple if you freeze it 3 minutes later?
C. Plot the decay of mRNA in your sample on a linear scale and on a log scale? 0.25 0.20 0.15 0.10 0.05 0.00 0.0 0.5 1.0 1.5 2.0 2.5 3.0 D. Write the equation for the curve in each plot. 0.25 0.20 0.15 0.10 0.0 0.5 1.0 1.5 2.0 2.5 3.0 Poll 1: The y-intercept is (linear plot): A) 0 B) 0.25 Poll 2: The y-intercept is (log plot): A) 0.25 B) -0.602
Transcribed Image Text:C. Plot the decay of mRNA in your sample on a linear scale and on a log scale? 0.25 0.20 0.15 0.10 0.05 0.00 0.0 0.5 1.0 1.5 2.0 2.5 3.0 D. Write the equation for the curve in each plot. 0.25 0.20 0.15 0.10 0.0 0.5 1.0 1.5 2.0 2.5 3.0 Poll 1: The y-intercept is (linear plot): A) 0 B) 0.25 Poll 2: The y-intercept is (log plot): A) 0.25 B) -0.602
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