OBSERVATIONS AND INTERPRETATIONS1 Record your observations in the table below.temitayo Cellular Morphology and Arrangement(include a written description and a detailed sketchof a few representative cells)StainOrganismand DurationCell Dimensions Rhodospirillum rubrum safranin for 60 seconds. Dimensions: 3.0-10.0 um x 0.8-1.0 µm in diameter475corbis

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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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OBSERVATIONS AND INTERPRETATIONS
1 Record your observations in the table below.
temitayo Cellular Morphology and Arrangement
(include a written description and a detailed sketch
of a few representative cells)
Stain
Organism
and Duration
Cell Dimensions

Rhodospirillum rubrum safranin for 60 seconds. Dimensions: 3.0-10.0 um x 0.8-1.0 µm in diameter
475
corbis

Expert Solution

Step 1

In a simple staining procedure, only one stain is used to stain the organism which produces a contrast when observed against an unstained background. The stains chosen for the simple staining technique are basic stains that positively charged chromogen so that it binds to the negatively charged nucleic acids and certain cell wall components of the organism. Examples of simple stains include methylene blue, crystal violet, and safranin. The morphology of the organism and the way in which they are arranged can be observed by a simple staining technique.

The procedure for simple staining technique is as follows:

Clean the microscopic slides and dry them properly.
Sterilize the inoculating loop and transfer a loopful of tap water on to the slide.
Sterilize the inoculating loop and transfer a loopful of the inoculum onto the slide.
Make a smear of the inoculum in the tap water.
Heat fix the smear by passing the underside of the slide through the flame 3 to 4 times.
Stain the slide with safranin and allow it to stay for 60 seconds.
Wash off the excess stain and dry the slide
Observe the slide under the microscope

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