[O2CC(O)CH2CO2] 2− + H2O → C2O44−+ CH3COO− + H+ oxaloacetate oxalic acid acetate In a 1-ml reaction mixture containing 50 mM NaCl, 50 mM HEPES, pH 7.8, 5 mM oxaloacetate and 5 pmol oxaloacetase, complete hydrolysis of the oxaloacetate was achieved in 10 minutes of incubation at 30°C. What was the pH of the reaction mixture at the end of the reaction?
HEPES, 4-(2-hydro)-1-piperazine ethane sulfonic acid, shown below. fully protonated, has pKa’s of 3.00 and 7.55. It is a commonly used buffer for tissue culture and organ storage during transplantation due in part to its effectiveness over a wide temperature range. One-way oxalic acid can be produced in
[O2CC(O)CH2CO2] 2− + H2O → C2O44−+ CH3COO− + H+
oxaloacetate oxalic acid acetate
In a 1-ml reaction mixture containing 50 mM NaCl, 50 mM HEPES, pH 7.8, 5 mM oxaloacetate and 5 pmol oxaloacetase, complete hydrolysis of the oxaloacetate was achieved in 10 minutes of incubation at 30°C.
What was the pH of the reaction mixture at the end of the reaction?
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