Molecules involved in DNA replication A. DNA Polymerase I B. DNA Polymerase II C. Single strand binding proteins D. Helicase E. DNA Ligase F. Primase Match each of the molecules with their specific role in DNA replication. NOTE there is only one correct molecule for each role. 1 Unwinds the helical structure of DNA and separates the two strands Prevents the two strands from reforming hydrogen bonds with each other 3 Synthesizes a short fragment of RNA to serve as a starting point for DNA replication Synthesizes most of the new DNA by using the complementary strand as a template 5 Replaces short RNA fragments with DNA Forms the final covalent bonds to make each strand of DNA a contiguous monomer of nucleotides

Molecules involved in DNA replication A. DNA Polymerase I B. DNA Polymerase II C. Single strand binding proteins D. Helicase E. DNA Ligase F. Primase Match each of the molecules with their specific role in DNA replication. NOTE there is only one correct molecule for each role. 1 Unwinds the helical structure of DNA and separates the two strands Prevents the two strands from reforming hydrogen bonds with each other 3 Synthesizes a short fragment of RNA to serve as a starting point for DNA replication Synthesizes most of the new DNA by using the complementary strand as a template 5 Replaces short RNA fragments with DNA Forms the final covalent bonds to make each strand of DNA a contiguous monomer of nucleotides

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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2) DNA replication in leading strand and lagging strand create inefficiency of replication speed because one is continuous synthesis and the other is discontinuous in opposite orientation away from the replication fork. Think about one possible way to overcome discrepancy of replication efficiency on both strands.
1. DNA replication is A. Conservative B. Semiconservative C. Dispersive D. A combination of the above 2. The Meselson-Stahl experiment was conducted to understand: A. The cell cycle phases B. mRNA translation C. DNA structure D. DNA replication 3. Which statement is true about DNA helicase? A. It hydrolyzes ATP B. It breaks the covalent bonds in the DNA C. It can move on the DNA in any direction (5'-to-3' or 3'-to-5') D. It is needed on both DNA strands
A drug that inhibits the DNAa protein is added to a culture of E. coli cells. What is the first step of DNA replication that will be impaired by this drug? PICK ONE ANSWER: A. Keeping parent DNA strands separated B. Releasing tension generated by supercoiling C. Activating the origin of replication D. Making primers E. Forming phosphodiester bonds
Which of the following enzymes is NOT involved in DNA replication? a. DNA Polymerase b. Primase c. Helicase d. Phosphatase e. DNA Ligase
2. This is responsible for breaking hydrogen bonds between complementary nucleotides of a DNA duplex during replication Group of answer choices 1. Topoisomerase 2. RNA Primase 3. Helicase 4. Single-Stranded Binding Protein
14. Guanine normally pairs with cytosine. Oxidative damage can result in the modification of guanine to 8-oxoguanine. What is the potential mismatch in nucleotides that can occur following replication? 8-oxoguanine pairs with Adenine 8-oxoguanine pairs with thymidine Thymidine pairs with adenine Guanine pairs with Cytosine None of these choices. Question 8 The key enzyme that transfers an a-amino group from an amino acid to an a-keto acid is : Glutamate Dehydrogenase Transaminase Synthetase Glutamine PRPP Amidotransferase Glutaminase
a.Write out the complementary strand to the following sequence. Begin with the 5' of the end of the complementary strand. 3 ' AGCAGTGACA5' b. Which of the following statements about DNA structure is correct? *The phosphodiester backbone of DNA is composed of covalent bonds between nudeotides * Two DNA strands in a double helix have their 5ends next to each other * Hydrogen bonds connect phosphate and hydroxyl groups on neighbouring nucleotides * The 5hydroxyl group on one nucleotide is linked to the 3 phosphate on its neighbor * In a double helix, purines pair with purines, and pyrimidines with pyrimidines C). Topoisomerases are enzymes that : * Create nucleosomes *Break phosphodiester bonds between DNA nucleotides * Synthesize a new strand of DNA from a single-stranded DNA template *Break hydrogen bonds between DNA nucleotides *Remove proteins from DNA
The single strand of DNA below forms a stem-loop structure structure with a loop that is 6 nucleotides long. 5’ - T A T C G C T A __ __ __ __ __ __ __ __ - 3’ What are the rest of the nucleotides? Group of answer choices a. 5’ – ATAGCATC – 3’ b. 5’ – CTACGATA – 3’ c. 5’ – AATCGTAG – 3’ d. 5’ – GATGCTAA – 3’
The leading strand in DNA replication requires only one primer for its synthesis. The lagging strand requires several primers because a. the lagging strand is prepared in short segments b. the lagging strand lacks a topoisomerase c. the leading strand serves as a template for the lagging strand d. All DNA polymerases read from the 5′ end.
Match to their function from list.
4) Replication of a circular DNA molecule can occur by either theta replication or by rolling circle replication. Describe or explain three differences between these two modes of DNA replication (you must accurately describe the differences between the two - an accurate statement about one form of replication that does not clearly indicate how the other form differs will not answer the qestion in it's entirety).
A student mixes various molecule needed for DNA replication. When he adds DNA, replication occurs, but each DNA duplex consists of a normal DNA strand paired with numerous segments of DNA a few hundred nucleotides long. What has been left out of the mixture? A. NTPs B. DNA polymerase III C. ATP D. DNA polymerase I E. DNA ligase