microbiology lab experiment: Identification of unkown bacterial cultures Q) Provide a specific example of an application of bacterial identification
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Q) Provide a specific example of an application of bacterial identification
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- microbial sensivity lab: in the procedure used to test bacterial growth against various temperatures (incubator, room, refrigerator, freezer), why should efforts be made to inoculate each tube with the same number of bacteria?The techniques for identifying unknown bacteria can be summarized in three key steps, explain in your own words how to: Staining the unknown for initial characterization by microscopy. Using a dichotomous key strategy to systematically rule out other organisms. Testing the organism for key biochemical traits.Provide the function of the following in blotting techniques: Nitrocellulose paper paper weight Probe Washing steps Visualization
- MALDI-TOF, is a method for identifying bacteria quickly. Look at this graphic then put the steps in order. Please follow a pathway of steps involving the Bacterial Culture: Get a readout of species identification and (sometimes) whether it is resistant or susceptible to antibiotics. Use colony from a nutrient agar plate. Prepare the sample, run it through MALDI-TOF machine. Grow a clinical sample on nutrient agar.procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar plate to agar slant.Discuss the functions of the following components in performing gel electrophoresis; Polyacrylamide gel Power supply TBE buffer solution Restriction enzymes
- procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slantConsidering microbial identification methods, to which category would the following method belong based on what it measures? * Examination of the shape and height of bacterial colonies growing on a plate Phenotypic Genotypic Serological BiochemicalWhat can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.
- during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.You are givena mix culture of S. aureus, E. coli and P. aeruginosa. Besides using the streak plate method what other methods could you use to separate the bacteria? Please state what methods you would use, the result you would be looking for and interpret the result.Compare and contrast bacteriocidal, bacteriostatic and bacteriolytic agents. What are their effects on the optical density (OD) and viable count of a bacterial culture, respectively?