- [methyl-1“C]Pyruvate was administered to isolated liver cells in the presence of sufficient malonate to block succinate dehydrogenase completely. After a time, isocitrate was isolated and found to contain label in both carbon 2 and carbon 5: 14CH,-Coo HC-COo H14C-coo OH How do you explain this result?

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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- [methyl-1“C]Pyruvate was administered to isolated liver cells in the
presence of sufficient malonate to block succinate dehydrogenase
completely. After a time, isocitrate was isolated and found to contain
label in both carbon 2 and carbon 5:
14CH,-Coo
HC-COo
H14C-coo
OH
How do you explain this result?
Transcribed Image Text:- [methyl-1“C]Pyruvate was administered to isolated liver cells in the presence of sufficient malonate to block succinate dehydrogenase completely. After a time, isocitrate was isolated and found to contain label in both carbon 2 and carbon 5: 14CH,-Coo HC-COo H14C-coo OH How do you explain this result?
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