Match the peak chromatogram and the possible compound based on the information given (example: Peak A – Compound A). All peaks originated from one HPLC injection. 0.14 Peak 1 Peak 2 0.12 Peak 3 0.10- 1564 0.08 1730 1345 Peak 4 0.06- Peak A 0.04 1311 Peak B 1211 0.02- 567 0.00- 1.00 4.50 250 min 0.00 0.50 1.50 200 300 3.50 4.00 5.00 a) Mode: Normal phase HPLC Peaks: 1, 2, 3 and 4 Compounds mix: Aspartame; Benzoic Acid; Caffeine; Sorbic Acid NH 2 ОН HO, HO, Benzoic acid Caffeine Sorbic Acid Aspartame

Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Match the peak chromatogram and the possible compound based on the
information given (example: Peak A – Compound A). All peaks originated from
one HPLC injection.
0.14
Peak 1
Peak 2
0.12-
Peak 3
0.10-
1564
0.08-
1730
1345
Peak 4
0.06-
Peak A
1311
0.04
Рeak B
567
1211
0.02-
0.00-
0.00
0.50
1.00
1.50
2.00
3.00
3.50
4.00
5.00
250
min
4.50
(a) Mode: Normal phase HPLC
Peaks: 1, 2, 3 and 4
Compounds mix: Aspartame; Benzoic Acid; Caffeine; Sorbic Acid
NH 2
H
ОН
ОН
N'
Benzoic acid
Caffeine
Sorbic Acid
Aspartame
Transcribed Image Text:Match the peak chromatogram and the possible compound based on the information given (example: Peak A – Compound A). All peaks originated from one HPLC injection. 0.14 Peak 1 Peak 2 0.12- Peak 3 0.10- 1564 0.08- 1730 1345 Peak 4 0.06- Peak A 1311 0.04 Рeak B 567 1211 0.02- 0.00- 0.00 0.50 1.00 1.50 2.00 3.00 3.50 4.00 5.00 250 min 4.50 (a) Mode: Normal phase HPLC Peaks: 1, 2, 3 and 4 Compounds mix: Aspartame; Benzoic Acid; Caffeine; Sorbic Acid NH 2 H ОН ОН N' Benzoic acid Caffeine Sorbic Acid Aspartame
(b) Mode: Reverse phase HPLC
Peaks: 1, 2, 3 and 4
Compounds mix: Lipid, carbohydrate, peptide, oligopeptide
ОН ОН
OH
НО
ОН
ОН
carbohydrate
lipid
Но-
HO
H2N,
,.ι ΝΗ 2
HO
NH 2
OH
NH
NH
H2N°
H
H2N'
'N.
H
ОН
peptide
oligopeptide
O IZ
IZ
Transcribed Image Text:(b) Mode: Reverse phase HPLC Peaks: 1, 2, 3 and 4 Compounds mix: Lipid, carbohydrate, peptide, oligopeptide ОН ОН OH НО ОН ОН carbohydrate lipid Но- HO H2N, ,.ι ΝΗ 2 HO NH 2 OH NH NH H2N° H H2N' 'N. H ОН peptide oligopeptide O IZ IZ
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