mat Donot osl Infedion, production ofr now phages, and lysis An occasional Pliysate phage will contan a plece of the báctertal chromosome. The steps in a cotransduction experiment. The donor strain, which is arg+ met+ strs (able to synthesize arginine and methionine but sensitive to streptomycin), is infected with phage P1. Some of the cells are lysed by P1, and this P1 lysate is mixed with cells of the recipient strain, which are arg- met- strr. P1 phages in this lysate may carry fragments of the donor cell's chromosome, and the P1 phage may inject that DNA into the recipient cells. To identify recipient cells that have received the met+ gene from the donor strain, the recipient cells are plated on a growth medium that contains arginine and streptomycin but not methionine. To determine if the arg+ gene has also been cotransduced, cells from each bacterial colony (on the plates containing arginine and streptomycin) are lifted with a sterile wire loop and streaked on a medium that lacks both amino acids. If the cells can Mx PI lyate with rociplent cells that are ag mot-str New fask containing millons of redplont colls Occasionaly, a raciplent cel wi racaive ag andor met trom aPi phaga mot Reciplent cel Plate on a growth modum with arginine and streptomycin but without methionine grow, cotransduction of the arg+ and met+ genes has occurred. Alternatively, if the cells of the restreaked colony do not grow, they must have received only the met+ gene during transduction. 50 colonies Pick cach of the 50 colonies and restreak Only the restroaking of 5 colonies is shown Growth of bactertal cels Growth modium without arginine Genotype of cels arg" in each colony org met ag mot aig mot mot mot Results Number of colonies that grow on Seleded Norselocted gine Cotrarsdudtion gene foquency modium medum argnine 50 arginine 21 met" 0.42
mat Donot osl Infedion, production ofr now phages, and lysis An occasional Pliysate phage will contan a plece of the báctertal chromosome. The steps in a cotransduction experiment. The donor strain, which is arg+ met+ strs (able to synthesize arginine and methionine but sensitive to streptomycin), is infected with phage P1. Some of the cells are lysed by P1, and this P1 lysate is mixed with cells of the recipient strain, which are arg- met- strr. P1 phages in this lysate may carry fragments of the donor cell's chromosome, and the P1 phage may inject that DNA into the recipient cells. To identify recipient cells that have received the met+ gene from the donor strain, the recipient cells are plated on a growth medium that contains arginine and streptomycin but not methionine. To determine if the arg+ gene has also been cotransduced, cells from each bacterial colony (on the plates containing arginine and streptomycin) are lifted with a sterile wire loop and streaked on a medium that lacks both amino acids. If the cells can Mx PI lyate with rociplent cells that are ag mot-str New fask containing millons of redplont colls Occasionaly, a raciplent cel wi racaive ag andor met trom aPi phaga mot Reciplent cel Plate on a growth modum with arginine and streptomycin but without methionine grow, cotransduction of the arg+ and met+ genes has occurred. Alternatively, if the cells of the restreaked colony do not grow, they must have received only the met+ gene during transduction. 50 colonies Pick cach of the 50 colonies and restreak Only the restroaking of 5 colonies is shown Growth of bactertal cels Growth modium without arginine Genotype of cels arg" in each colony org met ag mot aig mot mot mot Results Number of colonies that grow on Seleded Norselocted gine Cotrarsdudtion gene foquency modium medum argnine 50 arginine 21 met" 0.42
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
If these two genes were very far apart on the bacterial chromosome, how would the results have been different?
Transcribed Image Text:mat
Donot osl
Infedion, production ofr
now phages, and lysis
An occasional
Pliysate
phage will contan
a plece of the
báctertal
chromosome.
The steps in a cotransduction experiment. The donor strain, which
is arg+ met+ strs (able to synthesize arginine and methionine but
sensitive to streptomycin), is infected with phage P1. Some of the
cells are lysed by P1, and this P1 lysate is mixed with cells of the
recipient strain, which are arg- met- strr. P1 phages in this lysate
may carry fragments of the donor cell's chromosome, and the P1 phage
may inject that DNA into the recipient cells. To identify recipient
cells that have received the met+ gene from the donor strain, the
recipient cells are plated on a growth medium that contains arginine
and streptomycin but not methionine. To determine if the arg+ gene has
also been cotransduced, cells from each bacterial colony (on the plates
containing arginine and streptomycin) are lifted with a sterile wire loop
and streaked on a medium that lacks both amino acids. If the cells can
Mx PI lyate
with rociplent
cells that are
ag mot-str
New fask containing
millons of redplont
colls
Occasionaly, a
raciplent cel wi
racaive ag
andor met trom
aPi phaga
mot
Reciplent cel
Plate on a growth
modum with arginine
and streptomycin but
without methionine
grow, cotransduction of the arg+ and met+ genes has occurred. Alternatively,
if the cells of the restreaked colony do not grow, they must have received
only the met+ gene during transduction.
50 colonies
Pick cach of the 50
colonies and restreak
Only the restroaking of
5 colonies is shown
Growth of
bactertal cels
Growth modium
without arginine
Genotype of cels arg"
in each colony
org
met
ag
mot
aig
mot
mot
mot
Results
Number of colonies
that grow on
Seleded
Norselocted
gine
Cotrarsdudtion
gene
foquency
modium
medum
argnine
50
arginine
21
met"
0.42
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