Lys345 abstracts a proton by general base catalysis. Two Mg2+ ions stabilize the resulting enolate intermediate. Glu211 facilitates elimination of the Mg2+ PO? Mg?+ PO -OH group by general acid catalysis. H он Mg²+- Mg2+: PO C-C-H OH OH НОН Enolase Но H но H-N-H H-N*-H 2 Lys345 Lys345 Glu211 Glu211 2-Phosphoglycerate bound to enzyme Enolate intermediate Phosphoenolpyruvate Figure 6-26a Lehninger Principles of Biochemistry, Sixth Edition © 2013 W. H. Freeman and Company =

Biochemistry
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Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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Chapter1: Biochemistry: An Evolving Science
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In what type of catalysis does Lys345 engage during the very first step of enolase's reaction cycle? Explain, in your own words, specifically why and how Lys345 falls into the category you chose.

Lys345 abstracts a
proton by general
base catalysis. Two
Mg2+ ions stabilize
the resulting enolate
intermediate.
Glu211 facilitates
elimination of the
Mg2+
PO?
Mg?+
PO
-OH group by
general acid
catalysis.
H
он
Mg²+-
Mg2+:
PO
C-C-H
OH
OH
НОН
Enolase
Но
H
но
H-N-H
H-N*-H
2
Lys345
Lys345
Glu211
Glu211
2-Phosphoglycerate bound to enzyme
Enolate intermediate
Phosphoenolpyruvate
Figure 6-26a
Lehninger Principles of Biochemistry, Sixth Edition
© 2013 W. H. Freeman and Company
=
Transcribed Image Text:Lys345 abstracts a proton by general base catalysis. Two Mg2+ ions stabilize the resulting enolate intermediate. Glu211 facilitates elimination of the Mg2+ PO? Mg?+ PO -OH group by general acid catalysis. H он Mg²+- Mg2+: PO C-C-H OH OH НОН Enolase Но H но H-N-H H-N*-H 2 Lys345 Lys345 Glu211 Glu211 2-Phosphoglycerate bound to enzyme Enolate intermediate Phosphoenolpyruvate Figure 6-26a Lehninger Principles of Biochemistry, Sixth Edition © 2013 W. H. Freeman and Company =
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