Loop containing sample 3 (a) Steps In a Streak Plate; this one is a four-part or quadrant streak. (b) O Kathy Park Talaro

Q: (1) What is the difference between specific growth rate and generation time?

A: As per our policy we are answering the first question only.  For the rest of the question kindly…

Q: There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one…

A: Let's say, the yeast cell culture which was incubated for 6  hours as  = sample A the yeast cell…

Q: A culture with approximately 3x104 cells/mL were incubated. After 3 hours, the number of cells had…

A: The number of generations (n) refers to the number of times a cell population got doubled during a…

Q: A student was observing a filament of blue green bacteria under low power (60X). The diameter of the…

A: A. Average length of one cell = 2.4 mm/ 40=0.06 mm Under 60X = 40 cells, each is 0.06 mm in length

Q: In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are…

A: Given,number of colonies in a 1mL of culture = 250. Also,given that this given solution is 100 times…

Q: Give two reasons as to why anyone would bother SYBRGreen (SG) staining samples that contain…

A: SYBR Green (SG) is an asymmetrical cyanine nucleic acid stain. It binds to DNA.

Q: Using one of the graphs obtained with the medium II, name the different phases of the bacterial…

A: Micro organisms are typically grown in a closed culture media. Nutrients are added according to the…

Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…

A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…

Q: You make a 1:100 dilution of your culture and plated 0.1 ml of this sample on a Nutrient agar plate.…

A: The amount of organisms present in the original culture is called as colony forming unit per…

Q: Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution…

A: Serial dilutions is a process of making step by step dilutions of a substance. It is formed by…

Q: A sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells…

A: The viable plate count, or simply plate count, is a count of viable or live cells. It is based on…

Q: How would I figure out how many dilutions to make given cell density ? And volume to plate ? For…

A: Serial dilutions (log dilutions) are used to reduce bacterial concentrations to the levels necessary…

Q: Did each handwashing treatment result in similar types of organisms growing on the plates? (look at…

A: The science of tiny creatures' biology includes viruses, bacterium, algal, fungus, slime moulds, and…

Q: Why is a metal loop heat to read before picking up a bacterial sample? What should you do with a…

A: In microbiology, inoculation is the technique of transferring bacteria into culture media so that…

Q: Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If…

A: Fibronectin is a glycoprotein found in the extracellular matrix of cells and is also obtained from…

Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: a. Explain whether or not any of the methods in fi gure 2.9 could beused to determine the total…

A: Since, the question a. in incomplete as it requires figure 2.9 to be answered we will answer the…

Q: Write instruction to make cell culture medium using DMEM consist of 10% FBS, 1% streptomycin.

A: Introduction Streptomycin is an antimycobacterial drug which is used in the treatment of…

Q: A microbiology student was given a mixed culture of two different gram positive bacteria species to…

A: In Microbiology, we grow bacteria on culture medium. Culture media contains specific mixture of…

Q: ake a look at your streak plate from the use of a mix culture. Does your plate show the results that…

A: The streak plate method is a microbiological technique used for the isolation of pure strain ;…

Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…

A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…

Q: You want to subculture your cells from T25 flask to 96-well plates. You first collected your cells…

A: A hemocytometer is an instrument that is commonly used to count the number of cells present in a…

Q: Three-dimensional images of specimens can be produced using z stacks obtained from confocal…

A: Microscopy is a technique that is used to visualize small-sized objects that are not used to the…

Q: You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell…

A: Hemocytometer is device used for the counting of blood cells in a sample. It has counting chambers…

Q: If you were using the quadrant streak plate method to plate a very dilute broth culture ( with many…

A: Quadrant streaking is a microbiological technique which is used for isolation of pure strain from…

Q: Refer to the provided image drawn by a student trying to plan out their serial dilution protocol.…

A: Colony forming unit (CFU)is a measure of viable bacterial cell. CFU/ml can be calculated using…

Q: Why is UV light used in Column chromatography experiment ?

A: A chromatography procedure used to separate a single chemical compound from a mixture is known as…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique used for staining the microbial samples. It was performed by the…

Q: Why is PBS buffer preferred when preparing a monolayer cell culture?

A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…

Q: Calculate the colony forming units (CFU) if 100  colonies grow in agar using  a 0.01-mL calibrated…

A: One colony equals to 100 col/mL in  0.01 -mL calibrated loop.

Q: A tampered sack of powdered milk was analyzed by getting 50 grams of composited sample and diluting…

A: Dilution : We know that the solute of the concentration will be decreasing in solution . There is a…

Q: You perform a set of serial dilutions using 99-mL water blanks (successive 1-mL dilutions into…

A: Colony forming units (CFUs) are used to determine the concentration of viable microorganisms in a…

Q: Voges Proskauer test Does color of the medium change to red after both reagents were added and…

A: Biochemical method of bacterial identification is a classical method that is used for the…

Q: What should be considered when preparing a monolayer cell culture?

A: Monolayer culture, also known as adherent culture, is a form of animal cell culture in which cells…

Q: In the preparation of a pure culture media, the petri plates are inverted when placed in an…

A: In microbiology, culture media is considered the growth medium that possesses the specific mixtures…

Q: You wish to centrifuge and pellet yeast cells using a centrifuge. The protocol says that you need to…

A: Centrifugation is the process by which particles in a solution are separated based on the density…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique to identify bacterial stain gram-positive or gram-negative. It can be…

Q: During the heat shock step, a student accidentally heated the cells at 42°C for 5 minutes. Only a…

A: The heat shock step is an important part of certain molecular biology techniques, such as…

Q: A serial dilution was performed for a viable count of bacteria. The original bacterial cuture…

A: Bacteria are unicellular organisms, they grow as colonies. The colonies can be visualized with the…

Q: How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume?…

A: Dilution factor refers to the ratio of the volume of the initial (concentrated) solution to the…

Q: A strep culture is sampled at two points in time, 1 hour and 10 minutes apart. 3.2 x 105 cells/ml…

A:

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.