Loop containing sample 3 (a) Steps In a Streak Plate; this one is a four-part or quadrant streak. (b) O Kathy Park Talaro

Q: There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one…

A: Let's say, the yeast cell culture which was incubated for 6  hours as  = sample A the yeast cell…

Q: A culture with approximately 3x104 cells/mL were incubated. After 3 hours, the number of cells had…

A: The number of generations (n) refers to the number of times a cell population got doubled during a…

Q: A student was observing a filament of blue green bacteria under low power (60X). The diameter of the…

A: A. Average length of one cell = 2.4 mm/ 40=0.06 mm Under 60X = 40 cells, each is 0.06 mm in length

Q: In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are…

A: Given,number of colonies in a 1mL of culture = 250. Also,given that this given solution is 100 times…

Q: Give two reasons as to why anyone would bother SYBRGreen (SG) staining samples that contain…

A: SYBR Green (SG) is an asymmetrical cyanine nucleic acid stain. It binds to DNA.

Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…

A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…

Q: You make a 1:100 dilution of your culture and plated 0.1 ml of this sample on a Nutrient agar plate.…

A: The amount of organisms present in the original culture is called as colony forming unit per…

Q: A sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells…

A: The viable plate count, or simply plate count, is a count of viable or live cells. It is based on…

Q: Did each handwashing treatment result in similar types of organisms growing on the plates? (look at…

A: The science of tiny creatures' biology includes viruses, bacterium, algal, fungus, slime moulds, and…

Q: Why is a metal loop heat to read before picking up a bacterial sample? What should you do with a…

A: In microbiology, inoculation is the technique of transferring bacteria into culture media so that…

Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: a. Explain whether or not any of the methods in fi gure 2.9 could beused to determine the total…

A: Since, the question a. in incomplete as it requires figure 2.9 to be answered we will answer the…

Q: A microbiology student was given a mixed culture of two different gram positive bacteria species to…

A: In Microbiology, we grow bacteria on culture medium. Culture media contains specific mixture of…

Q: ake a look at your streak plate from the use of a mix culture. Does your plate show the results that…

A: The streak plate method is a microbiological technique used for the isolation of pure strain ;…

Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…

A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…

Q: Three-dimensional images of specimens can be produced using z stacks obtained from confocal…

A: Microscopy is a technique that is used to visualize small-sized objects that are not used to the…

Q: You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell…

A: Hemocytometer is device used for the counting of blood cells in a sample. It has counting chambers…

Q: If you were using the quadrant streak plate method to plate a very dilute broth culture ( with many…

A: Quadrant streaking is a microbiological technique which is used for isolation of pure strain from…

Q: Refer to the provided image drawn by a student trying to plan out their serial dilution protocol.…

A: Colony forming unit (CFU)is a measure of viable bacterial cell. CFU/ml can be calculated using…

Q: Why is UV light used in Column chromatography experiment ?

A: A chromatography procedure used to separate a single chemical compound from a mixture is known as…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique used for staining the microbial samples. It was performed by the…

Q: You perform a set of serial dilutions using 99-mL water blanks (successive 1-mL dilutions into…

A: Colony forming units (CFUs) are used to determine the concentration of viable microorganisms in a…

Q: Voges Proskauer test Does color of the medium change to red after both reagents were added and…

A: Biochemical method of bacterial identification is a classical method that is used for the…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique to identify bacterial stain gram-positive or gram-negative. It can be…

Q: During the heat shock step, a student accidentally heated the cells at 42°C for 5 minutes. Only a…

A: The heat shock step is an important part of certain molecular biology techniques, such as…

Q: How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume?…

A: Dilution factor refers to the ratio of the volume of the initial (concentrated) solution to the…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.