List five (5) factors affecting fixation of the cells in a physical and chemical state

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
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List five (5) factors affecting fixation of the cells in a physical and chemical state

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Fixation:

Fixation is a procedure in which cells or tissue are fixed partially in a chemical and partially in a physical condition so they can withstand further treatment with different reagents with the least amount of loss, distortion, or decomposition. 

Fixatives function by precipitating or denaturing proteins, which then cross-link to form a meshwork. Insoluble proteins give the meshwork mechanical strength for following operations, and it tends to hold the other cell constituents in vivo in relation to one another.

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