Lisa and Jisoo wanted to prepare 150 mL of 0.650 M malonate buffer pH 5.00 by mixing NaC4H304 and Na2C3H2O4. Relevant Reaction: Ка, 1.4х10з Ка, 2.0х106 C;H,O4 2 CH;04" 2 G,H,0,²- 1. What mass of solid NaC3H3O4 (MM = 138.054 g/mol) and solid Na2C3H2O4 (MM = 148.026 g/mol) will be needed to prepare the buffer? a. mass of solid NaC3H3O4 needed: b. mass of solid Na2C3H2O4 needed:

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Chapter1: Biochemistry: An Evolving Science
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Lisa and Jisoo wanted to prepare 150 mL of 0.650 M malonate buffer pH 5.00 by mixing NaC4H304 and Na2C3H204.
Relevant Reaction:
Ка, 3 1.4x10з
Kaz = 2.0x10 6
C;H,O4
2 C,H;O4
1. What mass of solid NaC3H3O4 (MM = 138.054 g/mol) and solid Na2C3H2O4 (MM = 148.026 g/mol) will be needed to prepare the buffer?
a. mass of solid NaC3H3O4 needed:
b. mass of solid NazC3H2O4 needed:
g
Transcribed Image Text:Lisa and Jisoo wanted to prepare 150 mL of 0.650 M malonate buffer pH 5.00 by mixing NaC4H304 and Na2C3H204. Relevant Reaction: Ка, 3 1.4x10з Kaz = 2.0x10 6 C;H,O4 2 C,H;O4 1. What mass of solid NaC3H3O4 (MM = 138.054 g/mol) and solid Na2C3H2O4 (MM = 148.026 g/mol) will be needed to prepare the buffer? a. mass of solid NaC3H3O4 needed: b. mass of solid NazC3H2O4 needed: g
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