MedialacZ expression+ glucose - lactose+ glucose + lactose- glucose - lactose- glucose + lactoseLowLowHighHighWhat components of the lac operon could be mutated to produce thisphenotype? List all possibilities.

lac operon is an inducible operon. That is, the lac operon is generally turned off and it is turned…

Answered: lacZ expression Low Low High High Media…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Give the levels of Beta-galactosidase activity (high or low or zero) expected for the following partial diploids for the lac operon strains.
For some time it was not clear whether lac repressor inhibits lac operon transcription by inhibiting the binding of RNA polymerase to its pro- moter or by allowing transcription initiation but blocking elongation past the site of bound repressor. How might you distinguish between these possibilities?
amount of protein / amount of mRNA 12 mm / ↑ A B time (minutes) 20 Bacteria were grown in culture. At various time points, substances were added or removed from the culture medium. To assay the effects of the substances on operon gene expression, samples of bacteria were collected at one-minute intervals and levels of specific proteins and mRNAs were determined. If the operon under investigation is a repressible operon, which arrow indicates the addition of a molecule that binds the repressor protein?
Operons A. Draw a schematic of the lac operon when glucose is low and lactose is high in the cell’s environment. B. Draw a schematic of the trp operon when tryptophan levels in the environment are low. C. What is the lux operon and how is affected by quorum sensing?
Like the lac operon, the hexose operon is controlled by a separate regulatory protein under the control of its own promoter (see the schematic of the operon below). The hexose regulatory protein is sensitive to fatty acyl CoA levels. When all hexose fuel sources are depleted, the bacteria switch to lipid metabolism and fatty acyl CoA levels increase. This turns expression of the hexose operon off. +1 +1 Regulatory Gene P regulator Hexose Operon Genes operon regulator promoter operon promoter e. The regulatory protein that controls expression of the hexose operons is a transcriptional ACTIVTOR or REPRESSOR (circle one).
Select all of the cis-acting regulatory elements of the lac operon. Operator CAP+cAMP CAP+cAMP binding site LacZ
Predict the growth/color pattern you'd expect for a mutant that can't activate both sugar operons (like the lac and mal operon). Table 1: Options are blue, white, or either for NA plates; Table 2: Options are: growth or none for minimal plates). Table 1 either Table 2 no growth Xgal glucose white Xgal + lactose growth Minimal lactose ΝΑ blue Xgal + IPTG growth maltose blu
The lac operon contains three genes: lacZ, lacY, and lacA for ß-galactosidase, galactoside permease, and thiogalactoside transacetylase, respectively. The operon also contains a promoter site and an operator site. The regulatory gene includes the I gene for the Lac repressor protein and a promoter for that gene. regulatory gene DNA P₁ lacl gene The lac operon undergoes negative regulation. In the normal condition, the Lac repressor protein is active. Allolactose, an isomer of lactose, is the signal molecule that binds to the Lac repressor. P O Increase in gene products The I gene is disabled. Lactose is present in the cell. The Lac repressor dissociates from DNA. lac operon lacZ gene Determine which events will increase the concentration of lac gene products and which will decrease the concentration of lac gene products. Allolactose is not present in the cell. lacy gene lacA gene Decrease in gene products Allolactose binds to the Lac repressor protein. The Lac repressor remains bound…
external lactose cell membrane RNA polymerase promoter lactose permease B-galactosidase allolactose lac repressor mRNA operator lacZ lacY lacA lac Operon The role of lac permease is to: Inhibit beta-galactosidase Facilitate entrance of lactose in to the bacterial cell Turn off the lac operon Manufacture mRNA
CAP-binding site Gene for Operator Gene for repressor protein Promoter for I gene Promoter for permease lac operon Gene for Gene for B-galactosidase transacetylase lac P, A Regulatory region Coding region For the above picture, draw all the proteins and molecules AND WHERE THEY BIND under conditions of no lactose, no glucose.
Distinguish between positive and negative control. Give examples of each from the lac operon
. Listed in parts a through g are some mutations that were found in the 5′ UTR of the trp operon of E. coli. What will the most likely effect of each of these mutations be on the transcription of the trp structural genes? Q. A mutation that prevents the binding of the ribosome to the 5′ end of the mRNA 5′ UTR

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lacZ expression Low Low High High Media + glucose - lactose + glucose + lactose - glucose - lactose - glucose + lactose What components of the lac operon could be mutated to produce this phenotype? List all possibilities.