Lab: Isolation of beta-amylase from sweet potato Objective: To isolate the enzyme β -amylase using sweet potato as a source Materials Required: Sweet potato. Knife/peeler. Mortar and Pestle. A Blender. Blue capped tubes. 20mM sodium Phosphate buffer at pH 7. Vortexer. Procedure: 1.Take a clean sweet potato and peel the skin off. 2.Weigh the peeled sweet potato and note the weight. 3.The sweet potato is cut into small pieces and transferred into a mortar and pestle. 4.The pieces are crushed and then transferred into a blender. 5.Add 40 ml of cold 20mM sodium phosphate buffer saline. Blend it until it forms a paste. 6.Gently transfer the potato slurry into a blue capped tube. 7.Allow the enzyme to extract over a 1 hour period at room temperature, with frequent vigorous stirring on a vortex mixer. 8.Then the extract is filtered using a GF A glass fibre filter and the filtrate is collected in a new blue capped tube. 9.Centrifuge the filtrate at 12000rpm for 20 minutes at 4 degree Celsius. 10. After the centrifugation, take out the blue capped tube. 11. Carefully transfer the supernatant into a new blue capped tube and is stored at 4 degree Celsius in refrigerator and discard the pellet. From the infor above plz answer these 2 questions 1. What is the principle of this experiment 2. What will you observed when the isolated beta amylase enzyme is subjected to biuret test
Lab: Isolation of beta-amylase from sweet potato
Objective:
To isolate the enzyme β -amylase using sweet potato as a source
Materials Required:
Sweet potato.
Knife/peeler.
Mortar and Pestle.
A Blender.
Blue capped tubes.
20mM sodium Phosphate buffer at pH 7.
Vortexer.
Procedure:
1.Take a clean sweet potato and peel the skin off.
2.Weigh the peeled sweet potato and note the weight.
3.The sweet potato is cut into small pieces and transferred into a mortar and pestle.
4.The pieces are crushed and then transferred into a blender.
5.Add 40 ml of cold 20mM sodium phosphate buffer saline. Blend it until it forms a paste.
6.Gently transfer the potato slurry into a blue capped tube.
7.Allow the enzyme to extract over a 1 hour period at room temperature, with frequent vigorous stirring on a vortex mixer.
8.Then the extract is filtered using a GF A glass fibre filter and the filtrate is collected in a new blue capped tube.
9.Centrifuge the filtrate at 12000rpm for 20 minutes at 4 degree Celsius.
10. After the centrifugation, take out the blue capped tube.
11. Carefully transfer the supernatant into a new blue capped tube and is stored at 4 degree Celsius in refrigerator and discard the pellet.
From the infor above plz answer these 2 questions
1. What is the principle of this experiment
2. What will you observed when the isolated beta amylase enzyme is subjected to biuret test
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